色谱

色谱

• 研究简报 • 上一篇    下一篇

高效液相色谱法测定家兔血浆中桂皮酸的浓度及其药代动力学初探

王睿,孙天慧,景丹,陈晓辉,于治国,毕开顺   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2004-07-08 修回日期:1900-01-01 出版日期:2005-05-30 发布日期:1988-09-25
  • 通讯作者: 毕开顺
  • 基金资助:

High Performance Liquid Chromatographic Determination of Cinnamic Acid in Rabbit Plasma and

WANG Rui, SUN Tianhui, JING Dan, CHEN Xiaohui, YU Zhiguo, BI Kaishun   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2004-07-08 Revised:1900-01-01 Online:2005-05-30 Published:1988-09-25

PDF

587

被引次数

22

摘要： 建立了采用高效液相色谱(HPLC)测定家兔血浆中桂皮酸含量的方法,并应用此法进行了桂皮酸的药代动力学研究。 采用的色谱柱为Kromasil C18柱(250 mm×4.6 mm i.d.,5 μm)；流动相为甲醇-乙腈-水-冰醋酸(体积比为10∶22∶55 ∶0.5),流速0.8 mL/min；检测波长为270 nm;柱温为室温；内标物为苯丙酸。实验结果表明,低、中、高浓度的提取回 收率分别为84.9%,84.4%,87.7%,方法回收率分别为98.4%,99.2%,100.1%,相对标准偏差(RSD)分别为5.5%,3.6%,3.7%, 日内及日间测定值的RSD均小于6%。所建立的HPLC方法灵敏、专一、准确、精密,可作为桂皮酸在家兔体内药代动力学研 究的检测手段。口服冠心苏合丸和冠心苏合胶囊后,桂皮酸在家兔体内的代谢呈一级吸收双室模型。

关键词: 高效液相色谱法, 冠心苏合胶囊 , 冠心苏合丸, 桂皮酸, 药代动力学

Abstract: A high performance liquid chromatographic method was developed to determine the content of cinnamic acid in rabbit plasma and the method was utilized for the study of pharmacokinetics. Cinnamic acid was separated by employing a column of Kromasil C18 (250 mm×4.6 mm i.d., 5 μm) and a mobile phase of methanol-acetonitrile-water-glacial acetic acid (10∶22∶55∶0.5, v/v) at a flow rate of 0.8 mL/min and room temperature with UV detection at 270 nm and phenylpropionic acid as internal standard. The extraction recoveries of the spiked samples at low, middle and high levels were 84.9%, 84.4%and 87.7%, respectively, while the method recoveries were 98.4% with relative standard deviation (RSD) of 5.5%, 99.2% with RSD of 3.6%, 100.1% with RSD of 3.7% in turn. The RSDs of intra-day and inter-day were both lower than 6%. Finally, the metabolism of cinnamic acid in rabbit plasma after medication of Guanxin Suhe Wan and Guanxin Suhe Capsule fitted in a first order absorption of two-compartment model. The method was found to be sensitive, accurate and precise, and is appropriate for the determination of cinnamic acid.

Key words: Guanxin Suhe , cinnamic acid, pharmacokinetics, high performance liquid chromatography