色谱

色谱

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高效液相色谱法对黄精多糖相对分子质量及组成的测定

张晓红,博·格日勒图,昭日格图,娜日苏   

  1. Institute of Macromolecular Chemistry and Mongolian Medicines, Inner Mongolia University, Huhhot 010021, China
  • 收稿日期:2004-07-09 修回日期:1900-01-01 出版日期:2005-07-30 发布日期:1988-06-25
  • 通讯作者: 张晓红
  • 基金资助:

Determination of Relative Molecular Mass and Composition for Polygonatum sibiricum Polysaccharide by

ZHANG Xiaohong, Gereltu Borjihan, Zhaorigetu, Narisu   

  1. Institute of Macromolecular Chemistry and Mongolian Medicines, Inner Mongolia University, Huhhot 010021, China
  • Received:2004-07-09 Revised:1900-01-01 Online:2005-07-30 Published:1988-06-25

PDF

1038

被引次数

48

摘要： 从内蒙古野生黄精根茎中提取出粗黄精多糖,并用胰蛋白酶法和Sevag法结合脱去其中的蛋白质，采用高效液相色谱法,利用Shodex GPC色谱柱对其纯度、相对分子质量和相对分子质量的分布进行了测定,结果表明所得产物为均一成分,测得的数均相对分子质量（Mn）为7073,相对分子质量（Mr）为7247,分散系数为1.025。以水为流动相,利用Shodex SP0810糖色谱柱对黄精多糖的组成进行分析,结果表明,黄精多糖是由单一的果糖组成的。

关键词: 高效液相色谱法, 黄精多糖 , 相对分子质量, 组成

Abstract: Polygonatum sibiricum Redoute (PSR) is a traditional Chinese medicine and Mongolia herbal medicine growing in Inner Mongolia. Polygonatum Sibiricum Polysaccharide (PSP) was isolated and purified with boiling water extraction, alcohol precipitation from the wild species roots of PSR. Proteins was removed from the crude PSP by methods of trypsin hydrolysate and Sevag. Purity, relative molecular mass and relative molecular mass distribution of PSP were determined using high performance liquid chromatography (HPLC). The buffer solution of NaH2PO4-Na2HPO4 (0.02 mol/L) was used as mobile phase, and the PSP was separated on a Shodex GPC column as a single symmetrical peak corresponding to a number mean molecular mass (Mn) of 7073 and relative molecular mass (Mr) of 7247, and polydispersity is 1.025. The sugar components of the PSP was analysed by HPLC and 13C NMR. Using water as mobile phase, the components of all acid hydrolysis of the PSP were separated on a Shodex SP0810 column. Glucose, galactose, mannose and fructose were used as the standard samples. The result showed that PSP is composed of fructose only.

Key words: composition, Polygonatum sibiricum polysaccharide , relative molecular mass, high performance liquid chromatography