色谱

色谱

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高效液相色谱法测定山楂中的苦杏仁甙

吕伟峰,丁明玉

  

  1. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education,
    Department of Chemistry, Tsinghua University, Beijing 100084, China
  • 收稿日期:2005-05-13 修回日期:1900-01-01 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 丁明玉

Determination of Amygdalin in Hawthorn by High Performance Liquid Chromatography

Lv Weifeng, DING Mingyu   

  1. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education,
    Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2005-05-13 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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摘要：

建立了从山楂中提取苦杏仁甙的方法,样品先用石油醚脱脂，然后用甲醇进行索氏提取。用高效液相色谱法定量测定了山楂中的苦杏仁甙,色谱条件如下：反相C18柱,流动相为15%的甲醇水溶液,检测波长为215 nm。测定了含不同比例山楂籽的山楂样品,结果表明含山楂籽比例高的山楂样品中苦杏仁甙的含量高,且山楂粗粒样品中苦杏仁甙的提取量比粉末样品的提取量高。

关键词: 超声提取, 高效液相色谱法, 苦杏仁甙, 山楂
, 索氏提取

Abstract:

A suitable method for extraction of amygdalin from hawthorn has been established. At first, the lipophilic components were removed with petroleum ether by ultrasonic extraction. The amygdalin was then extracted by methanol in a Soxhlet’s apparatus. For quantitation, a high performance liquid chromatographic method was developed by using a reversed-phase C18 column, mobile phase of methanol-water (15∶85, v/v) and a detection wavelengh of 215 nm. It can be concluded that the content of amygdalin is higher in the seeds than that in the hawthorn powder without the seeds and the yield of amygdalin is higher in the hawthorn pieces than that in the hawthorn powder.

Key words: amygdalin, hawthorn , Soxhlet extraction, ultrasonic extraction, high performance liquid chromatography