色谱

色谱

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应用高效液相色谱-二极管阵列检测器-蒸发光散射检测器联用技术同时测定清开灵注射液中的五类有效成分

严诗楷1,2,辛文峰1,3,罗国安1,王义明1,2,程翼宇2

  

  1. 1. Department of Chemistry, Tsinghua University, Beijing 100084, China; 2. Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China; 3. Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China
  • 收稿日期:2005-06-03 修回日期:1900-01-01 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 罗国安

Simultaneous Determination of Five Groups of Components in Qingkailing Injection by High Performance Liquid Chromatography with Photo Diode Array Detector and Evaporative Light Scattering Detector

YAN Shikai1,2, XIN Wenfeng1,3, LUO Guoan1, WANG Yiming1,2, CHENG Yiyu2   

  1. 1. Department of Chemistry, Tsinghua University, Beijing 100084, China; 2. Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China; 3. Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China
  • Received:2005-06-03 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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摘要：

建立了复方清开灵注射液中5类主要有效成分的定量测定方法。应用高效液相色谱-二极管阵列检测器-蒸发光散射检测器联用技术(HPLC-DAD-ELSD),根据各类成分紫外吸收光谱的差异,分别在240,254,280和330 nm波长下检测栀子苷、核苷(包括尿苷和腺苷)、黄芩苷和有机酸(包括绿原酸和咖啡酸)等4类成分,同时使用ELSD测定胆酸、熊去氧胆酸和猪去氧胆酸等3种甾体化合物,从而实现了清开灵注射液中5类有效成分(共9个化合物)的同时分离和定量测定。用该法测定了3个不同厂家的19批清开灵注射液成品。该法快速、准确，操作相对简单,为中药复方复杂体系的多组分定量测定和质量控制提供了一种可靠、合理且简便、易行的方法模式。

关键词: 二极管阵列检测器, 高效液相色谱法, 清开灵注射液 , 有效成分, 蒸发光散射检测器

Abstract:

A method was established for the simultaneous quantification of nine components of five different structural types in Qingkailing injection. High performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector (HPLC-DAD-ELSD) was employed in the determination. Four monitoring wavelengths of 240, 254, 280 and 330 nm were set to determine nucleosides (uridine and adenosine), iridoid glucoside (geniposide), flavone glycoside (baicalin) and organic acids (chlorogenic acid and caffeic acid) respectively, and a combined evaporative light scattering detector was used to detect three steroid compounds (cholic acid, ursodesoxycholic acid and hyodeoxycholic acid). The proposed method permitted the simultaneous separation and determination of five groups of compounds in Qingkailing injection, and acceptable validation results of the precision, repeatability, stability and accuracy tests were achieved. The method was applied to the analysis of 19 Qingkailing injection samples from three different plants, and the results indicated that the method could be used as a convenient and reliable method in the multi-component determination and quality control of traditional Chinese medicines.

Key words: active constituents, evaporative light scattering detector, photo diode array detector, Qingkailing injection , high performance liquid chromatography