色谱

色谱

• 研究简报 • 上一篇    下一篇

中药菟丝子中生物活性成分的毛细管电泳-电化学检测

傅亮,楚清脆,管月清,叶建农

  

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • 收稿日期:2004-09-20 修回日期:1900-01-01 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 叶建农

Determination of Bioactive Components in Semencustae by Capillary Electrophoresis with Electrochemical Detection

FU Liang, CHU Qingcui, GUAN Yueqing, YE Jiannong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:2004-09-20 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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摘要：

采用毛细管电泳-电化学检测法(CE-ECD)同时测定了菟丝子中芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素等5种主要生物活性成分的含量,考察了运行缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离检测的影响。在最佳实验条件下,以直径300 μm的碳圆盘电极为工作电极,检测电位为+950 mV(vs. 参比电极),以50 mmol/L的硼砂缓冲溶液(pH 9.0)为运行缓冲液,上述各组分在19 min内能完全分离。芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素在两个数量级的范围内呈良好线性关系,检测下限(按S/N=3计) 分别为1.93×10-5，3.55×10-4，3.65×10-5,1.73×10-5和1.46×10-4 g/L。该法已成功地应用于菟丝子中活性成分的分离检测,结果令人满意。

关键词: 电化学检测, 对香豆酸, 槲皮素, 金丝桃甙, 芦丁, 毛细管电泳, 山柰酚, 菟丝子, 中药

Abstract:

A method of high performance capillary electrophoresis with electrochemical detection (CE-ECD) has been developed for the determination of five bioactive components in Semencustae, namely rutin, hyperoside, kaempferol, p-coumaric acid and quercetin. The effects of several factors such as the acidity and concentration of the running buffer, the separation voltage, the applied potential and the injection time on CE-ECD were investigated. Under the optimized conditions, these five components can be separated in a 50.0 mmol/L borax running buffer (pH 9.0) within 19 minutes. A 300 μm diameter carbon disk electrode was used as the working electrode positioned carefully opposite to the outlet of the capillary in a wall-jet configuration at potential of +950 mV (vs. saturated calomel electrode as reference electrode, SCE). Good linear relationships were established between the peak current and concentration of analytes over two orders of magnitude. The detection limits (S/N=3) were 1.93×10-5, 3.55×10-4, 3.65×10-5, 1.73×10-5 and 1.46×10-4 g/L for rutin, hyperoside, kaempferol, p-coumaric acid and quercetin, respectively. The method has been successfully applied to the determination of these analytes in Semencustae samples after a relatively simple extraction procedure, and the assay results were satisfactory.

Key words: electrochemical detection； rutin, hyperoside, kaempferol, p-coumaric acid, quercetin, Semencustae, traditional Chinese medicine , capillary electrophoresis