色谱

色谱

• 研究简报 • 上一篇    下一篇

凝胶柱净化-高效液相色谱检测食品中的苏丹红

谢维平,黄盈煜,傅晖蓉,胡桂莲

  

  1. Quanzhou Sanitary and Anti-Epidemic Station, Quanzhou 362000, China
  • 收稿日期:2005-03-28 修回日期:2005-06-07 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 谢维平

Simultaneous Determination of Sudan Red Dyes in Foods by High Performance Liquid Chromatography with
a Clean-Up Procedure by Gel Column

XIE Weiping, HUANG Yingyu, FU Huirong, HU Guilian   

  1. Quanzhou Sanitary and Anti-Epidemic Station, Quanzhou 362000, China
  • Received:2005-03-28 Revised:2005-06-07 Online:2005-09-30 Published:1988-03-25

PDF

939

摘要：

建立了凝胶柱净化-高效液相色谱同时检测食品中苏丹红Ⅰ,Ⅱ,Ⅲ和Ⅳ的方法。样品用乙醇提取,提取液经Bio-Beads SX3凝胶柱(200 mm×10 mm i.d.)净化,用环己烷-乙酸乙酯(体积比为1∶1)洗脱。采用Symmetry Shield RP18柱(250 mm×4.6 mm i.d.,　5　μm)分离,以100%甲醇为流动相,流速1.5 mL/min;用二极管阵列检测器检测,检测波长478 nm。上述4种苏丹红组分在其质量浓度为0.1~10.0 mg/L时有良好的线性关系(r>0.999),方法的检测限为7~14 μg/kg;平均加标回收率为80.7%~96.3%(添加水平为0.25,2.5 mg/kg),相对标准偏差为2.4%~5.9%。方法灵敏可靠,能满足食品中苏丹红检测的需要。

关键词: 高效液相色谱, 凝胶柱, 食品 , 苏丹红

Abstract:

A method was developed for the simultaneous determination of Sudan Red Ⅰ, Ⅱ, Ⅲ and Ⅳ in foods by high performance liquid chromatography (HPLC) with a clean-up procedure by gel column. Sample was extracted from foods with ethanol. The extract was cleaned up with a Bio-Beads SX3 gel column (200 mm×10 mm i.d.) and eluted with cyclohexane-ethyl acetate (1∶1, v/v). The analysis was performed on a Symmetry Shield RP18 column (250 mm×4.6 mm i.d., 5 μm) with 100% methanol as the mobile phase at a flow rate of 1.5 mL/min, detection at 478 nm and confirmation by diode-array spectra. All of the four compounds demonstrated good linear relationship (r>0.999) in the range of 0.1-10.0 mg/L. The limits of detection (LOD) were from 7 to 14 μg/kg. The average recoveries for all four dyes (spiked at the levels of 0.25 and 2.5 mg/kg) in chili sauce and sausage ranged from 80.7% to 96.3%, and the relative standard deviations were from 2.4% to 5.9%. The method is sensitive, reliable and can be applied for the analysis of four Sudan Red dyes in foods.

Key words: food , high performance liquid chromatography, Sudan Red dyes, gel column