色谱

色谱

• 研究简报 • 上一篇    下一篇

高效液相色谱法分析环境样品中的苯基胂化合物

李善茂,李伟,岳丽君,左伯莉

  

  1. Analytical Center, Institute of Chemical Defence, Beijing 102205, China
  • 收稿日期:2004-09-21 修回日期:1900-01-01 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 李伟

Determination of Phenylarsenic Compounds in Environmental Samples by High Performance Liquid Chromatography

LI Shanmao, LI Wei, YUE Lijun, ZUO Boli   

  1. Analytical Center, Institute of Chemical Defence, Beijing 102205, China
  • Received:2004-09-21 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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712

摘要：

二苯氯胂和二苯氰胂是刺激性毒剂,在环境中易于降解,其产物苯胂酸、苯胂氧、二苯胂酸、氧联双二苯胂和三苯胂比较稳定,对环境危害大。建立了同时测定这5种含胂产物的反相高效液相色谱方法,选择了最佳色谱条件,提供了各组分的紫外光谱图。5种化合物的线性范围分别为8~30,5~40,20~4000,120~8000,1~60 mg/L,检测限分别为0.1,0.1,0.2,10,0.1 mg/L。对实际环境样品进行了分析,结果较好。

关键词: 苯基胂化合物
, 苯胂酸, 苯胂氧, 二苯胂酸, 高效液相色谱法, 三苯胂, 氧联双二苯胂

Abstract:

A reversed-phase high performance liquid chromatographic method was developed for simultaneous determination of phenylarsonic acid (PAA), phenylarsine oxide (PAO), diphenylarsinic acid (DPAA), bis(diphenylarsine)oxide (BDPAO) and triphenylarsine (TPA) in soil samples. The extraction solvent for soil samples was acetonitrile and ultrasound was used in the extraction. Good separation was achieved by using an Inertsil CN-3 column (250 mm×4.6 mm i.d., 5 μm) with the following conditions: gradient program of mobile phase, a 10 min linear gradient from 100% A (a mixture of acetonitrile-2.5 g/L KH2PO4 (pH 2.0) buffer (5∶95, v/v) ) to 100% B ( a mixture of acetonitrile-2.5 g/L KH2PO4 (pH 2.0) buffer (50∶50, v/v) ), and isocratic at 100% B from 10.01 min to 28 min; flow rate of 1.0 mL/min; time-programmed UV detection at 210 nm (0-10.5 min, 20-28 min) and 268 nm (10.5-20 min). The linear range of PAA, PAO, DPAA, BDPAO and TPA were 8-30, 5-40, 20-4000, 120-8000 and 1-60 mg/L, respectively. The detection limits at a signal-to-noise ratio of 3 were 0.1, 0.1, 0.2, 10, and 0.1 mg/L for PAA, PAO, DPAA, BDPAO and TPA, respectively.

Key words: bis(diphenylarsine)oxide, diphenylarsinic acid, phenylarsenic compounds , phenylarsine oxide, phenylarsonic acid, triphenylarsine, high performance liquid chromatography