色谱

色谱

• 研究简报 • 上一篇    下一篇

高效阴离子交换色谱在易极化阴离子痕量分析中的应用

墨淑敏1,2,梁立娜1,蔡亚岐1,牟世芬1,温美娟2

  

  1. 1. State Key Laboratory of Environmental Chemistry and Ecotoxicity, Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, Beijing 100085, China; 2. Department of Chemistry, University of Science and Technology Beijing, Beijing 100083, China
  • 收稿日期:2004-11-01 修回日期:1900-01-01 出版日期:2005-11-30 发布日期:1987-12-25
  • 通讯作者: 牟世芬

Application of High Performance Anion Exchange Chromatography for Trace Analysis of Polarizable Anions

MO Shumin1,2, LIANG Lina1, CAI Yaqi1, MOU Shifen1, WEN Meijuan2   

  1. 1. State Key Laboratory of Environmental Chemistry and Ecotoxicity, Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, Beijing 100085, China; 2. Department of Chemistry, University of Science and Technology Beijing, Beijing 100083, China
  • Received:2004-11-01 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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摘要：

以45 mmol/L的 NaOH溶液作淋洗液,采用强亲水性的IonPac AS16阴离子交换分析柱和脉冲安培检测器,在15 min内分离检测了Br-,S2O2-3,I-和SCN-4种易极化阴离子,检测限(以3倍信噪比计)分别为0.5,0.2,0.05和2 μg/L (进样25 μL)。痕量离子的标准溶液平行9次进样测定的相对标准偏差为0.8%～3.7%。而在相同的色谱条件下,抑制型电导检测器对上述4种阴离子的检测限分别为1,1,2和10 μg/L,平行9次进样测定的相对标准偏差为0.9%～4.7%。通过实验比较可知,脉冲安培检测器的灵敏度是抑制型电导检测器的2～40倍。在测定上述易极化阴离子时,脉冲安培检测器具有选择性强、精密度好和灵敏度高等优点。

关键词: 高效阴离子交换色谱, 脉冲安培检测器, 抑制型电导检测器 , 易极化阴离子

Abstract:

Polarizable anions such as Br-, S2O2-3, I- and SCN- were separated using 45 mmol/L sodium hydroxide solution as the mobile phase on a high hydrophilic IonPac AS16 column. With a pulsed amperometric detector, the detection limits were 0.5, 0.2, 0.05 and 2 μg/L (25.0 μL injected, signal-to-noise ratio of 3) for Br-, S2O2-3, I- and SCN-. The relative standard deviation (RSD) range of trace anions was from 0.8% to 3.7% (n=9). Under the same chromatographic conditions, these anions were also determined using a suppressed conductivity detector and the detection limits were 1, 1, 2 and 10 μg/L (25 μL injected, signal-to-noise ratio of 3), respectively. The RSD range was from 0.9% to 4.7% (n=9). Comparing a pulsed amperometric detector with a conductivity detector, the former is 2 to 40 times more sensitive than the latter. For the determination of polarizable anions, a pulsed amperometric detector has higher selectivity, precision and sensitivity.

Key words: polarizable anions, pulsed amperometric detector, suppressed conductivity detectorI
, high performance anion exchange chromatography