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色谱

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毛细管电泳法研究环糊精与药物芦丁和氯霉素的相互作用

傅崇岗,杨冬芝

  

  1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China
  • 收稿日期:2004-11-29 修回日期:1900-01-01 出版日期:2005-11-30 发布日期:1987-12-25
  • 通讯作者: 傅崇岗

Investigation on Interactions of Rutin and Chloramphenicol with β-Cyclodextrin Using Capillary Electrophoresis

FU Chonggang, YANG Dongzhi   

  1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China
  • Received:2004-11-29 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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摘要：

采用毛细管电泳淌度移动法测定了β-环糊精与芦丁和氯霉素这两种药物的包合常数及包合反应的热力学参数。所用未涂层熔融石英毛细管的规格为内径50 μm,总长37 cm,有效长度30 cm，电泳缓冲液为25 mmol/L硼砂+100 mmol/L硼酸(pH 8.6),并加入不同浓度的β-CD。在25 ℃时测得芦丁和氯霉素与β-CD的包合常数分别为98.5和1204 L/mol。通过测定不同温度下的包合常数,求得包合反应的系列热力学参数。结果表明β-CD与两种药物的包合反应焓熵互补现象明显。采用MM2力场对β-CD与两种药物分子的包合物的稳定构型进行了分子力学模拟,模拟结果与实验结果相符。

关键词: β-环糊精, 包合常数, 芦丁, 氯霉素, 毛细管电泳, 热力学参数

Abstract:

The interactions of two medicines of rutin and chloramphenicol with β-cyclodextrin were investigated using mobility shift method by capillary electrophoresis. The size of the capillary column was 37 cm×50 μm i.d. with an effective length of 30 cm. The running buffer was a mixture of 25 mmol/L sodium borate and 100 mmol/L boric acid (pH 8.6). Effective mobilities of the two medicines changed with altering the concentration of β-cyclodextrin. By nonlinear least square fitting curve, the inclusion constants of 98.5 and 1204 L/mol for rutin and chloramphenicol were obtained at 25 ℃, respectively. The inclusion constants at different temperatures were determined, from which a series of thermodynamic parameters were derived. Experimental results show that an obvious relationship of mutual compensation between enthalpy and entropy existed in these inclusion reactions. The most stable configurations of the inclusions were calculated using MM2 force field, and the results were well in agreement with the experimental results.

Key words: chloramphenicol, inclusion constants, rutin, thermodynamic parameters , β-cyclodextrin, capillary electrophoresis