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Porous Monoliths: Stationary Phases of Choice for High Performance Liquid Chromatography in Various Formats

SVEC Frantisek

  

  1. Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA
  • 收稿日期:2005-09-08 修回日期:1900-01-01 出版日期:2005-11-30 发布日期:1987-12-25
  • 通讯作者: SVEC Frantisek

Porous Monoliths: Stationary Phases of Choice for High Performance Liquid Chromatography in Various Formats

SVEC Frantisek   

  1. Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA
  • Received:2005-09-08 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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摘要：

Modern porous monoliths have been conceived as a new class of stationary phases for high performance liquid chromatography (HPLC) in classical columns in the early 1990s and later extended to the capillary format. These monolithic materials are prepared using simple processes carried out in an external mold (inorganic monoliths) or within the confines of the column (organic monoliths and all capillary columns). These methods afford macroporous materials with large through-pores that enable applications in a rapid flow-through mode. Since all the mobile phase must flow through the monolith, the convection considerably accelerates mass transport within the monolithic separation medium and improves the separations. As a result, the monolithic columns perform well even at very high flow rates. The applications of monolithic capillary columns are demonstrated on numerous separations in the HPLC mode.

关键词: porous monoliths, silica , stationary phases, synthetic polymers, high performance liquid chromatography

Abstract:

Modern porous monoliths have been conceived as a new class of stationary phases for high performance liquid chromatography (HPLC) in classical columns in the early 1990s and later extended to the capillary format. These monolithic materials are prepared using simple processes carried out in an external mold (inorganic monoliths) or within the confines of the column (organic monoliths and all capillary columns). These methods afford macroporous materials with large through-pores that enable applications in a rapid flow-through mode. Since all the mobile phase must flow through the monolith, the convection considerably accelerates mass transport within the monolithic separation medium and improves the separations. As a result, the monolithic columns perform well even at very high flow rates. The applications of monolithic capillary columns are demonstrated on numerous separations in the HPLC mode.

Key words: porous monoliths, silica , stationary phases, synthetic polymers, high performance liquid chromatography