色谱

色谱

• 研究论文 • 上一篇    下一篇

两种新型反相高效液相色谱填料疏水选择性能的比较

葛晋1,2,张红丽1,李秀娟1,2,邵士俊1,李永民1, 陈立仁1

  

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;
    2.Graduated School of the Chinese Academy of Sciences, Beijing 100039, China

  • 收稿日期:2005-08-02 修回日期:2005-11-15 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者:

    陈立仁

Comparison in Hydrophobic Selectivity for Two Types of Reversed-Phase High Performance Liquid
Chromatography Packing Material

GE Jin1,2, ZHANG Hongli1, LI Xiujuan1,2, SHAO Shijun1, LI Yongmin1, CHEN Liren1

  

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;
    2.Graduated School of the Chinese Academy of Sciences, Beijing 100039, China

  • Received:2005-08-02 Revised:2005-11-15 Online:2006-07-30 Published:1986-12-25
  • Contact: Chen Liren

PDF

684

摘要：

以层接层自组装法制备的TiO2/SiO2和ZrO2/SiO2微球为基质,分别与十八烷基三氯硅烷在甲苯溶液中加热回流36 h
制备了两种碳十八键合固定相。两种固定相的含碳量分别为11.51%和9.62%。对两种固定相的作用机理进行研究,并以9
种芳香类化合物对其色谱性能进行评价。结果表明，两种固定相都是较好的反相固定相,其疏水选择性差异不大,对芳香
性化合物均有较好的选择性,TiO2/SiO2的含碳量略高于ZrO2/SiO2。

关键词: 高效液相色谱法, 硅胶 , 碳十八键合固定相, 氧化锆, 氧化钛

Abstract:

Two types of novel reversed-phase packing materials were made by adding TiO2/SiO2 (or
ZrO2/SiO2) and octadecyltrichlorosilane into toluene with stirring and refluxing for 36 h. TiO2/SiO2
and ZrO2/SiO2 particles were prepared by a layer-by-layer self-assemble technique and consist of
micrometer-sized silica spheres as cores and nanometer-sized zirconia or titania as surface coating.
The carbon loading of C18-bonded TiO2/SiO2 was 11.51% and that of C18-bonded ZrO2/SiO2 was 9.62%.
The hydrophobic selectivity and sensitivity were studied respectively and compared in details; the
results showed that the packing supports both acted as true reversed chromatographic stationary
phase with similar hydrophobic selectivity. The chromatographic behaviors of the two types of novel
reversed-phase packing material were evaluated with nine aromatic compounds. The chromatographic
studies showed that either of the packing materials would be a good choice to separate aromatic
compounds, however, the selectivity of C18-bonded TiO2/SiO2 was a little better than that of
C18-bonded ZrO2/SiO2.

Key words: octadecyl-bonded stationary phase, silica , titania
(TiO2), zirconia (ZrO2), high performance liquid chromatography (HPLC)