色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱-蒸发光散射检测法测定蛋黄卵磷脂的含量

龚雁1,王巧娥2,杨屹1,丁明玉2

  

  1. 1.Department of Science, Beijing University of Chemical Technology, Beijing 100029, China;
    2.Key Lab of Bioorganic Phosphorous Chemistry & Chemical Biology, Ministry of Education,
    Department of Chemistry, Tsinghua University, Beijing 100084, China

  • 收稿日期:2005-09-27 修回日期:2006-02-09 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 丁明玉

Determination of Egg-Yolk Phosphatidylcholine by Normal-Phase High Performance Liquid Chromatography
with Evaporative Light Scattering Detection

GONG Yan1, WANG Qiao’e2, YANG Yi1, DING Mingyu2

  

  1. 1.Department of Science, Beijing University of Chemical Technology, Beijing 100029, China;
    2.Key Lab of Bioorganic Phosphorous Chemistry & Chemical Biology, Ministry of Education,
    Department of Chemistry, Tsinghua University, Beijing 100084, China

  • Received:2005-09-27 Revised:2006-02-09 Online:2006-07-30 Published:1986-12-25
  • Contact: DING Mingyu

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摘要：

建立了蛋黄磷脂中卵磷脂（即磷脂酰胆碱,PC)的高效液相色谱-蒸发光散射检测（HPLC-ELSD）的测定方法。以Nov
a-Pak Silica 60A硅胶柱(3.9 mm i.d.×150 mm,4 μm)为分离柱,正己烷-异丙醇-3%冰醋酸水溶液(体积比为35∶65
∶8)为流动相，等度洗脱,流速1.0 mL/min,柱温30 ℃。蒸发光散射检测器漂移管温度50 ℃,雾化气(空气)压力350
kPa。在上述条件下测得PC在0.16~1.61 g/L范围内线性关系良好(r2=0.9979),检测限为0.64
μg,方法的精密度为3.2%(n=5),回收率为98.2%~128.2%。将该方法用于实际样品的测定,获得了令人满意的结果。该方
法预处理简单,分析速度快,可用于蛋黄磷脂中卵磷脂的测定。

关键词: 蛋黄卵磷脂 , 高效液相色谱法, 磷脂酰胆碱, 蒸发光散射检测

Abstract:

A method for the determination of egg-yolk phosphatidylcholine by normal-phase high
performance liquid chromatography (NP-HPLC) with evaporative light scattering detection (ELSD) was
developed. Yolk phosphatidylcholine was successfully separated on a Nova-Pak Silica 60A column (3.9
mm i.d.×150 mm, 4 μm), using hexane-isopropanol-water containing 3% acetic acid (35∶65∶8, v/v/v)
as mobile phase at a flow rate of 1.0 mL/min. The drift tube temperature and air carrier gas
pressure of the ELSD were set at 50 ℃ and 350 kPa, respectively. The linear detection range was
0.16-1.61 g/L (r2=0.9979) and the limit of detection (LOD) was 0.64 μg with excellent relative
standard deviation (RSD) of 3.2% (n=5). The recovery was in the range of 98.2%-128.2%. The results
indicated that this method is accurate, rapid, simple and reproducible, and thus suitable for the
analysis of egg-yolk phosphatidylcholine in real samples.

Key words: egg-yolk phosphatidylcholine (EYPC) , evaporative light scattering detection
(ELSD), phosphatidylcholine (PC), high performance liquid chromatography (HPLC)