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毛细管区带电泳法测定卷烟中的生物碱

许庆平,何友昭

  

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China

  • 收稿日期:2005-08-08 修回日期:2005-12-03 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 许庆平

Determination of Alkaloids in Cigarettes by Capillary Zone Electrophoresis

XU Qingping, HE Youzhao

  

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China

  • Received:2005-08-08 Revised:2005-12-03 Online:2006-07-30 Published:1986-12-25
  • Contact: XU Qingping

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摘要：

在磷酸缓冲体系中采用毛细管区带电泳法测定卷烟中的生物碱时,检测灵敏度低,分离度差。考察了卷烟中生物碱的
提取条件，分离缓冲溶液的类型、pH值和浓度，卷烟中生物碱测定方法的线性范围、检出限、重现性和回收率。结果发
现，当采用410 mmol/L的酒石酸溶液（pH 2.8）为缓冲体系时,卷烟中生物碱的检测灵敏度和分离度均有明显改善,烟碱
的线性范围为0.06~0.80 mg/L（其他生物碱为0.006~0.10 mg/L）,检出限为0.002~0.01
mg/L,相对标准偏差为2.2%~10%,回收率为87.6%~102%。

关键词: 酒石酸缓冲溶液 , 卷烟, 毛细管区带电泳法, 生物碱

Abstract:

The separation of cigarette alkaloids was reported and performed with tartaric acid buffer
solution by capillary zone electrophoresis (CZE). A buffer solution of pH 2.8, 410 mmol/L tartaric
acid was used as the running buffer for the determination of cigarette alkaloids in CZE. The
detection sensitivity and resolution with the tartaric acid buffer solution were better than those
with phosphate buffer. The sample extraction conditions, buffer electrolyte, pH and concentrations
were investigated for the alkaloids separation and determination. The linear range, detection
limits, reproducibilities and recoveries of the cigarette alkaloids were 0.06-0.80 mg/L for
nicotine (0.006-0.10 mg/L for other alkaloids), 0.002-0.01 mg/L, 2.2%-10% and 87.6%-102%,
respectively.

Key words: alkaloid, cigarettes, tartaric acid buffer solution , capillary zone electrophoresis (CZE)