色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法制备纯化大豆异黄酮糖苷

杨学东1,邓志成1,4,王晶2,丁明玉3

  

  1. 1.College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China;
    2.National Research
    Center for CRM’S, Beijing 100013, China; 3.Department of Chemistry, Tsinghua University, Beijing
    100084,
    China; 4.Nanotechnology Industrialization Base of China, Tianjin 300457, China
  • 收稿日期:2005-08-17 修回日期:2006-01-29 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 邓志成

Preparation of Soybean Isoflavone Glucosides by Reversed-Phase High Performance Liquid
Chromatography

YANG Xuedong1, DENG Zhicheng1,4, WANG Jing2, DING Mingyu3   

  1. 1.College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China;
    2.National Research
    Center for CRM’S, Beijing 100013, China; 3.Department of Chemistry, Tsinghua University, Beijing
    100084,
    China; 4.Nanotechnology Industrialization Base of China, Tianjin 300457, China
  • Received:2005-08-17 Revised:2006-01-29 Online:2006-07-30 Published:1986-12-25

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摘要：

利用制备高效液相色谱法从大豆总异黄酮提取物中制备出了3种大豆异黄酮糖苷。在Nova-Pak HR C18色谱柱(100
mm×25 mm i.d.,6 μm)上,以甲醇-体积分数为0.1%的乙酸水溶液(体积比为23∶77)为流动相,流速为20 mL/min,采用
等度洗脱方式,制备了3种大豆异黄酮糖苷,经质谱分析,确认它们分别为大豆苷、黄豆苷和染料木苷。高效液相色谱分析
表明,所制备的3种化合物的纯度均达到了99%以上。

关键词: 大豆 , 大豆异黄酮, 葡萄糖苷, 制备高效液相色谱法

Abstract:

A method was established for the isolation of soybean isoflavone glucosides from the total
isoflavone extracts of soybean using preparative reversed-phase high performance liquid
chromatography (RP-HPLC). The total isoflavone extracts were separated into four parts by solvent
extraction, those are the ethyl acetate extract, butanol extract, precipitate (D4), and the
remaining aqueous phase. The part D4 containing soybean isoflavone glucosides was acquired and
subjected to preparative HPLC for the isolation of target components. A preparative Nova-Pak HR C18
column (100 mm×25 mm i.d., 6 μm) was used in the preparation process. By isocratic elution with
methanol-0.1% aqueous acetic acid (23∶77, v/v) as the mobile phase at a flow rate of 20 mL/min,
followed by concentration and desalination, three soybean isoflavone glucosides were obtained and
subsequently identified by mass spectrometry as daidzin, glycitin, and genistin. HPLC analysis
showed that the purities of the three soybean isoflavone glucosides were all higher than 99%.

Key words:
soybean , glucosides, soybean isoflavones, preparative high performance liquid chromatography