色谱

色谱

• 研究论文 • 上一篇    下一篇

银黄口服液的质量控制及其高效液相色谱指纹图谱的研究

王丽聪1,曹玉华1,徐红兰1,叶建农2

  

  1. 1.School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China;
    2.Department of Chemistry, East China Normal University, Shanghai 200062, China

  • 收稿日期:2005-08-08 修回日期:2006-03-09 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 曹玉华

Study on Quality Control and Fingerprint of Chinese Traditional Medicine Yinhuang Oral Liquid

WANG Licong1, CAO Yuhua1, XU Honglan1, YE Jiannong2

  

  1. 1.School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China;
    2.Department of Chemistry, East China Normal University, Shanghai 200062, China

  • Received:2005-08-08 Revised:2006-03-09 Online:2006-07-30 Published:1986-12-25
  • Contact: CAO Yuhua

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摘要：

利用高效液相色谱方法,建立了复方银黄口服液及其原料药材黄芩和金银花的指纹图谱,测定了银黄口服液中黄芩苷
和绿原酸的含量。以Lichrospher C18色谱柱(250 mm×4.6 mm i.d.,5 μm)为分离柱,以甲醇和0.1%磷酸水溶液为流动
相进行二元梯度洗脱,流速0. 6 mL/min,在254 nm波长下检测。采用夹角余弦和相关系数的方法,计算6批银黄口服液指
纹图谱的相似度均在0.99以上,表明银黄口服液的质量未见显著差异。将银黄口服液指纹图谱中的共有峰与原料药材黄芩
和金银花的指纹图谱中的共有峰相比较,对其归属进行确认,大部分共有峰可以匹配。

关键词: 高效液相色谱法, 黄芩, 金银花 , 银黄口服液, 指纹图谱

Abstract:

The chromatographic fingerprint of Chinese traditional medicine, Yinhuang Oral Liquid
together with its original materials Radix Scutellariae and Flos lonicera was established by high
performance liquid chromatography (HPLC). At the same time, baicalin and chlorogenic acid in the
Yinhuang Oral Liquid were also quantitatively determined. The analysis was performed on a
Lichrospher C18 column (250 mm×4.6 mm i.d., 5 μm) and with gradient elution. The optimum
conditions were explored. The mobile phase A was methanol and the mobile phase B was 0.1%H3PO4
aqueous solution. The wavelength of 254 nm was selected for detection. Finally, the data of
fingerprints of 6 samples of Yinhuang Oral Liquid were processed with two different mathematic
methods. This proposed method demonstrated good stability for the 6 samples of Yinhuang Oral Liquid.
In the comparison of the common peaks in chromatogram of Yinhuang Oral Liquid with those of crude
herbs, most of them could be matched well.

Key words: fingerprint, Flos lonicera , Radix
Scutellariae, Yinhuang Oral Liquid, high performance liquid chromatography （HPLC）