色谱

色谱

• 研究论文 • 上一篇    下一篇

葛根提取物高效液相色谱分析方法的优化

张岩,薛兴亚,徐青,章飞芳,梁鑫淼

  

  1. Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China

  • 收稿日期:2006-01-13 修回日期:2006-04-30 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者:

    梁鑫淼

Optimization of High Performance Liquid Chromatographic Method for Analysis of Kudzu Root Crude
Extract

ZHANG Yan, XUE Xingya, XU Qing, ZHANG Feifang, LIANG Xinmiao

  

  1. Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China

  • Received:2006-01-13 Revised:2006-04-30 Online:2006-07-30 Published:1986-12-25
  • Contact: LIANG Xinmiao

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摘要：

针对葛根提取物的复杂体系,分别从色谱模式、流动相、添加剂以及梯度洗脱条件几个方面对其高效液相色谱分析
方法进行了优化。其中梯度洗脱条件的优化是根据各组分在5次线性梯度下的保留时间并借助于CSASS软件快速准确地计
算出各组分的液相色谱保留参数a,c值以及峰形参数σ和τ,在此基础上使用CSASS软件对葛根提取物的色谱分离情况进行
高精度仿真预测,从而优化了梯度洗脱条件。考察了优化条件下的分析方法的精密度和重现性,结果表明该方法稳定、可
靠、重现性好。

关键词: CSASS软件, 高效液相色谱法, 葛根 , 优化

Abstract:

Kudzu root is an important traditional Chinese herb. Its crude extract has been used in
the treatment of hypertension and angina pectoris in China. Several parameters for high performance
liquid chromatography (HPLC) analytical method for kudzu root extract, including HPLC model, mobile
phase, additive and gradient conditions have been optimized. The HPLC retention parameters a and c,
and the peak shape parameters σ and τ of 25 chromatographic peaks were obtained accurately and
rapidly using five linear gradients and were calculated using CSASS software. The CSASS software
was then used to simulate the gradient conditions in the experiments, and the optimized condition
was obtained. The comparison of the simulated and real chromatogram profiles showed that the
potential of simulation using the CSSASS software as compared to that of the real experimental
conditions was precise. Reversed-phase HPLC model and the mobile phase of (A) acetonitrile
containing 0.1% (v/v) acetic acid and (B) water containing 0.1% (v/v) acetic acid were used. The
optimized gradient profile showed a linear increase as follows: from 5% A to 35% A at 0-33 min; from
35% A to 100% A at 33-50 min, and then the HPLC system was held for 5 min. The reproducibility and
precision of the method were investigated. The relative standard deviations (RSDs) of retention
time, peak height, and peak area were less than 0.25%, 11%, and 4.9%, respectively, which showed
that the method was stable, reliable, and reproducible.

Key words: CSASS software, kudzu root , optimize, high performance liquid chromatography (HPLC)