色谱

色谱

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高效液相色谱-荧光检测法测定土壤中的多环芳烃

钱薇,倪进治，骆永明,李秀华,邹德勋   

  1. State Key Laboratory of Soil and Sustainable Agriculture, Soil and Environment Bioremediation Research Center, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
  • 收稿日期:2006-06-05 修回日期:2006-11-15 出版日期:2007-03-30 发布日期:1985-12-25
  • 通讯作者: 骆永明
  • 基金资助:

Determination of Polycyclic Aromatic Hydrocarbons in Soil by High Performance Liquid Chromatography with Fluorescence Detection

QIAN Wei, NI Jinzhi, LUO Yongming, LI Xiuhua, ZOU Dexun   

  1. State Key Laboratory of Soil and Sustainable Agriculture, Soil and Environment Bioremediation Research Center, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
  • Received:2006-06-05 Revised:2006-11-15 Online:2007-03-30 Published:1985-12-25
  • Contact: LUO Yongming

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摘要： 多环芳烃(简称PAHs)是一类具有致癌、致畸以及能够诱导有机体突变的环境有机污染物。可靠的PAHs检测方法是研究其环境行为的重要保证。由于高效液相色谱-荧光检测法具有不需要高温、对某些PAHs有较高的分辨率和高灵敏度、柱后流出组分便于收集进行光谱鉴定等优点,近年来被广泛应用于PAHs的检测。实验在对美国环保局(USEPA)优先监测的15种PAHs污染物在土壤中的含量进行测定时，重点优化了梯度洗脱程序和检测波长程序。优化后的方法对15种PAHs的最低检出限为0.12～1.57 μg/kg,回收率为73%～126%,相对标准偏差为0.53%～3.57%。结果表明,该方法用于测定土壤中PAHs的含量,具有检出限低、灵敏度高和重复性好等优点,是一个较为可靠的检测方法。

关键词: 多环芳烃, 高效液相色谱法, 土壤 , 荧光检测

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are the environmental organic pollutants which can induce cancers, teratogeny and organism saltation. Therefore, a reliable detection method is essential for studying the environmentally hazardous PAHs. The high performance liquid chromatography with a highly sensitive fluorescence detector was introduced to determine 15 PAHs of the priority pollutants named by the US Environmental Protection Agency (EPA) in soil. The gradient elution procedure was improved and the detection wavelength was optimized. The detection limits and the recoveries of the improved method for the 15 PAHs were in the ranges of 0.12 μg/kg to 1.57 μg/kg and 73% to 126%, respectively. The relative standard deviations of this method were in the range of 0.53% to 3.57%. The results indicated that this method is a reliable detection method with low detection limit, high sensitivity and repeatability for the determination of PAHs in soil.

Key words: fluorescence detection, polycyclic aromatic hydrocarbons (PAHs), soil , high performance liquid chromatography (HPLC)