Chinese Journal of Chromatography

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Determination of Seven Biogenic Amines in Fish Using Micellar Electrokinetic Capillary Chromatography

GAN Ning1, LI Tianhua1, WANG Luyan1, JIANG Qianli2


  1. 1.Faculty of Material Science and Engineering, Ningbo University, Ningbo 315211, China;
    2.Blood Department, South Hospital, Guangzhou 510000, China
  • Received:2007-06-12 Revised:2007-08-21 Online:2007-11-30 Published:1984-12-25
  • Contact: Gan Ning


A method for the determination of histamine, 2-phenylethylamine, tryptamine, putrescine, cadaverine, spermidine and spermine in fish using micellar electrokinetic capillary chromatography (MECC) was developed. Seven biogenic amines (BAs) were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol (95∶5, v/v)was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges. The detection limit of histamine was 15 μg/g and those of the other amines were 5 μg/g. The developed method was successfully employed for the simultaneous determination of 7 BAs in fish sample with satisfactory results.

Key words: biogenic amines, fish , micellar electrokinetic capillary chromatography (MECC)