Chinese Journal of Chromatography ›› 2017, Vol. 35 ›› Issue (3): 252-254.DOI: 10.3724/SP.J.1123.2016.10001

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Specific recognition and detection of target proteins by new fluorescence molecularly imprinted membrane

ZHANG Xin1,2, JIANG Rui1, YANG Shu1, SUN Liquan1, PANG Siping2, LUO Aiqin1   

  1. 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
    2. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
  • Received:2016-10-04 Online:2017-03-08 Published:2013-07-16

Abstract:

A new method for producing L-cysteine-capped ZnS quantum dots (QDs) embedded molecularly imprinted membrane (QDs@MIM) was developed. The QDs@MIM was used as fluorescence artificial receptor for specific recognition and detection of target proteins. The QD@MIM was fabricated on a silylanized glass plate. Lysozyme, acrylamide, L-cysteine modified Mn2+-doped ZnS QDs and N,N'-methylenediacrylamide were used as template, functional monomer, assistant monomer and cross-linker, respectively. Under optimal conditions, the linear range for lysozyme ranged from 0.1 to 1.0 μmol/L, adsorption time was 4 min and the imprinting factor for lysozyme was 6.2. The results showed that the molecularly imprinted polymer can be used as a simple, rapid and selective biosensor to detect target proteins in biologic samples.

Key words: molecularly imprinted membranes (MIM), protein detection, quantum dots (QDs)

CLC Number: