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固定化牛血清白蛋白吸附材料的制备及其对胆红素的吸附性能

于志远1,2, 吴仁安1, 邹汉法1*   

  1. 1. 中国科学院分离分析化学重点试验室, 中国科学院大连化学物理研究所, 国家色谱研究分析中心, 辽宁 大连 116023; 2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2009-11-11 修回日期:2010-01-12 出版日期:2010-03-28 发布日期:1981-03-25
  • 通讯作者: 邹汉法

Preparation of bovine serum albumin immobilized adsorbent for specific adsorption of bilirubin

YU Zhiyuan1,2, WU Ren’an1, ZOU Hanfa1*   

  1. 1. CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2009-11-11 Revised:2010-01-12 Online:2010-03-28 Published:1981-03-25
  • Contact: ZOU Hanfa

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617

被引次数

11

摘要： 降低高胆红素血症和重症肝炎患者血液中异常升高的胆红素浓度是血浆交换和血液灌流等疗法的目标之一。本文通过共价键合的方法将牛血清白蛋白(BSA)固定在甲基丙烯酸缩水甘油酯共聚三羟甲基丙烷三甲基丙烯酸酯［poly(GMA-co-TMPTMA)］大孔树脂微球上,制备得到对胆红素具良好吸附性能的固定化BSA吸附材料(BIA),吸附容量达48.7 mg/g。由于血清白蛋白对胆红素的强烈相互作用,胆红素溶液中游离BSA的存在会显著降低BIA对胆红素的吸附量。BIA对胆红素的吸附量随吸附温度升高而增加。BIA在－80 ℃下储存31 d后性能仍然稳定,对胆红素的吸附量几乎不变。上述结果表明所制备的BIA为以特异性吸附胆红素为目的的血液净化材料提供了新的选择。

关键词: 胆红素 , 牛血清白蛋白, 微球, 吸附材料

Abstract: Serum bilirubin concentration is greatly elevated in certain diseases such as hyperbilirubinemia and severe hepatitis. Lowering the level of bilirubin is one of the major targets of many therapies such as plasma exchange and hemoperfusion. In this study, a bilirubin specific adsorbent was prepared by covalently immobilizing bovine serum albumin (BSA) onto macroporous poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) microspheres. The resulting BSA immobilized adsorbent (BIA) demonstrated good performance in adsorption of bilirubin with an adsorption capacity of 48.7 mg/g. Presence of BSA in bilirubin solution significantly lowered the adsorption capacity of the adsorbent due to the tight binding of bilirubin onto BSA. Adsorption performance of the adsorbent for bilirubin was improved with the elevation of temperature. The adsorbent demonstrated good stability even after 31 d of storage at －80 ℃ in that there was almost no change in adsorption capacity for bilirubin. These results indicated that the prepared BSA immobilized adsorbent could be an alternative choice for specific adsorption of bilirubin.

Key words: bilirubin , bovine serum albumin, microspheres, adsorbent