色谱

色谱 ›› 2015, Vol. 33 ›› Issue (1): 29-34.DOI: 10.3724/SP.J.1123.2014.08029

• 研究论文 • 上一篇    下一篇

Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection

Iman SAEIDI1, Behruz BARFI2, Moazameh PAYROVI3, Javad FEIZY4, Hojat A SHEIBANI5, Mina MIRI1, Farahnaz GHOLLASI MOUD1   

  1. 1. Iranian National Standards Organization, Mashhad 91735-344, Iran;
    2. Department of Chemistry, University of Semnan, Semnan 35131-19111, Iran;
    3. Department of Chemistry, University of Mazandran, Babolsar 47416-95447, Iran;
    4. Testa Quality Control Laboratory, Mashhad 91895/157-134, Iran;
    5. Birjand University of Technology, Birjand 97198-66981, Iran
  • 收稿日期:2014-08-27 修回日期:2014-11-04 出版日期:2015-01-08 发布日期:2014-12-26
  • 通讯作者: Iman SAEIDI
  • 基金资助:

    The authors are grateful to the financial support of Institute of Standards and Industrial Research of Iran (Khorasan-e-RazaviProvince).

Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection

Iman SAEIDI1, Behruz BARFI2, Moazameh PAYROVI3, Javad FEIZY4, Hojat A SHEIBANI5, Mina MIRI1, Farahnaz GHOLLASI MOUD1   

  1. 1. Iranian National Standards Organization, Mashhad 91735-344, Iran;
    2. Department of Chemistry, University of Semnan, Semnan 35131-19111, Iran;
    3. Department of Chemistry, University of Mazandran, Babolsar 47416-95447, Iran;
    4. Testa Quality Control Laboratory, Mashhad 91895/157-134, Iran;
    5. Birjand University of Technology, Birjand 97198-66981, Iran
  • Received:2014-08-27 Revised:2014-11-04 Online:2015-01-08 Published:2014-12-26
  • Supported by:

    The authors are grateful to the financial support of Institute of Standards and Industrial Research of Iran (Khorasan-e-RazaviProvince).

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摘要：

With polyamide (PA) as an efficient sorbent for solid phase extraction (SPE) of Sudan dyes II, III and Red 7B from saffron and urine, their determination by HPLC was performed. The optimum conditions for SPE were achieved using 7 mL methanol/water (1: 9, v/v, pH 7) as the washing solvent and 3 mL tetrahydrofuran for elution. Good clean-up and high (above 90%) recoveries were observed for all the analytes. The optimized mobile phase composition for HPLC analysis of these compounds was methanol-water (70: 30, v/v). The SPE parameters, such as the maximum loading capacity and breakthrough volume, were also determined for each analyte. The limits of detection (LODs), limits of quantification (LOQs), linear ranges and recoveries for the analytes were 4.6-6.6 μg/L, 13.0-19.8 μg/L, 13.0-5000 μg/L (r2 > 0.99) and 92.5%-113.4%, respectively. The precisions (RSDs) of the overall analytical procedure, estimated by five replicate measurements for Sudan II, III and Red 7B in saffron and urine samples were 2.3%, 1.8% and 3.6%, respectively. The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine samples with HPLC coupled with UV detection.

关键词: high-performance liquid chromatography (HPLC), polyamide (PA), saffron, solid phase extraction (SPE), Sudan dyes, urine

Abstract:

With polyamide (PA) as an efficient sorbent for solid phase extraction (SPE) of Sudan dyes II, III and Red 7B from saffron and urine, their determination by HPLC was performed. The optimum conditions for SPE were achieved using 7 mL methanol/water (1: 9, v/v, pH 7) as the washing solvent and 3 mL tetrahydrofuran for elution. Good clean-up and high (above 90%) recoveries were observed for all the analytes. The optimized mobile phase composition for HPLC analysis of these compounds was methanol-water (70: 30, v/v). The SPE parameters, such as the maximum loading capacity and breakthrough volume, were also determined for each analyte. The limits of detection (LODs), limits of quantification (LOQs), linear ranges and recoveries for the analytes were 4.6-6.6 μg/L, 13.0-19.8 μg/L, 13.0-5000 μg/L (r2 > 0.99) and 92.5%-113.4%, respectively. The precisions (RSDs) of the overall analytical procedure, estimated by five replicate measurements for Sudan II, III and Red 7B in saffron and urine samples were 2.3%, 1.8% and 3.6%, respectively. The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine samples with HPLC coupled with UV detection.

Key words: high-performance liquid chromatography (HPLC), polyamide (PA), saffron, solid phase extraction (SPE), Sudan dyes, urine

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