基于超高效液相色谱-高分辨质谱联用技术的桑黄干预后大鼠尿液代谢组学分析

doi:10.3724/SP.J.1123.2016.04009

色谱 ›› 2016, Vol. 34 ›› Issue (8): 811-816.DOI: 10.3724/SP.J.1123.2016.04009

基于超高效液相色谱-高分辨质谱联用技术的桑黄干预后大鼠尿液代谢组学分析

董宇¹, 俞忠明¹, 李洪玉¹, 赵丽沙², 寿旦¹

1. 浙江省中药新药研发重点实验室, 浙江省中医药研究院, 浙江杭州 310007;
2. 浙江中医药大学药学院, 浙江杭州 310053

收稿日期:2016-04-06 出版日期:2016-08-08 发布日期:2016-07-26
通讯作者: 寿旦
基金资助:
浙江省自然科学基金项目（LQ15H280008）；浙江省科技计划项目（2016F10027）.

Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry

DONG Yu¹, YU Zhongming¹, LI Hongyu¹, ZHAO Lisha², SHOU Dan¹

1. Key Laboratory of Research and Development of Traditional Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China;
2. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

Received:2016-04-06 Online:2016-08-08 Published:2016-07-26
Supported by:
Zhejiang Provincial Natural Science Foundation of China (No. LQ15H280008); Science and Technology Project of Zhejiang Province (No. 2016F10027).

摘要/Abstract

摘要：

为探讨服用桑黄水煎液对机体的影响，采用超高效液相色谱-高分辨质谱（UPLC-HDMS）联用技术，检测灌胃给予桑黄水煎液后大鼠尿液中代谢物的变化。采用正交偏最小二乘法判别分析（OPLS-DA）对空白组和给药组大鼠尿液代谢物进行聚类分析，筛选出潜在的生物标志物，并通过MetaboAnalyst 3.0网站分析相关代谢通路。数据显示，两组大鼠尿液中的代谢物在第28天得到了很好的区分，发现并鉴定了10个生物标记物。灌胃给予桑黄水煎液主要对机体的半胱氨酸和甲硫氨酸代谢、精氨酸和脯氨酸代谢、嘌呤代谢等代谢通路产生影响。研究结果为深入探讨桑黄药效作用机制奠定了一定的实验基础。

关键词: 超高效液相色谱, 大鼠尿液, 代谢组学, 高分辨质谱, 模式识别分析, 桑黄

Abstract:

To investigate the influence of oral administration of decoction of Phellinus igniarius on the body, this study utilized ultra high performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) technology to detect the change of metabolites in rat urine after oral decoction of Phellinus igniarius. Orthogonal partial least squares discriminant analysis (OPLS-DA) was adapted to analyze the metabolites of rat urine in the control group and dosed group, then the potential biomarkers were screened out. Finally the related metabolic pathways were analyzed by MetaboAnalyst 3.0 website. The results showed that urinary metabolites in the two groups were well separated at the 28th day, and 10 biomarkers were found and identified. After the oral administration of decoction of Phellinus igniarius, it mainly impacted the cysteine and methionine metabolism, arginine and proline metabolism, purine metabolism pathways. These results of the study would lay a foundation for further research on the pharmacodynamic mechanism of Phellinus igniarius.

Key words: Phellinus igniarius, high-definition mass spectrometry (HDMS), metabolomics, pattern recognition analysis, rat urine, ultra high performance liquid chromatography (UPLC)

中图分类号:

O658

董宇, 俞忠明, 李洪玉, 赵丽沙, 寿旦. 基于超高效液相色谱-高分辨质谱联用技术的桑黄干预后大鼠尿液代谢组学分析[J]. 色谱, 2016, 34(8): 811-816.

DONG Yu, YU Zhongming, LI Hongyu, ZHAO Lisha, SHOU Dan. Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(8): 811-816.

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基于超高效液相色谱-高分辨质谱联用技术的桑黄干预后大鼠尿液代谢组学分析

Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry

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