色谱

色谱 ›› 2016, Vol. 34 ›› Issue (10): 956-959.DOI: 10.3724/SP.J.1123.2016.05018

• 特别策划:离子态化合物色谱分析专栏 • 上一篇    下一篇

离子色谱-柱切换技术同步测定饮料中的三氯蔗糖、葡萄糖、果糖和蔗糖

陈宁1,2, 张培敏1, 吴述超3, 朱岩1,2   

  1. 1. 浙江大学化学系, 浙江 杭州 310028;
    2. 浙江省微量有毒化学物健康风险评估技术研究重点实验室, 浙江大学, 浙江 杭州 310028;
    3. 浙江省地质矿产研究所, 浙江 杭州 310007
  • 收稿日期:2016-05-26 出版日期:2016-10-08 发布日期:2013-04-03
  • 通讯作者: 朱岩
  • 基金资助:

    国家自然科学基金项目（21405141）；国家重大科学仪器设备开发专项（2012YQ09022903）；浙江省自然科学基金项目（LZ16B050001，LY15B050001，S16H180002）；浙江省微量有毒化学物健康风险评估技术研究重点实验室（2014006，2014007）；国土资源部黏土矿物重点实验室开放基金（2014-K03）.

Simultaneous determination of sucralose, glucose, fructose and sucrose in beverage by ion-exchange chromatography with column-switching

CHEN Ning1,2, ZHANG Peimin1, WU Shuchao3, ZHU Yan1,2   

  1. 1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China;
    2. Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Zhejiang University, Hangzhou 310028, China;
    3. Zhejiang Institute of Geology and Mineral Resources, Hangzhou 310007, China
  • Received:2016-05-26 Online:2016-10-08 Published:2013-04-03
  • Supported by:

    National Natural Science Foundation of China (No. 21405141); National Important Project on Science Instrument (No. 2012YQ09022903); Zhejiang Provincial Natural Science Foundation of China (Nos. LZ16B050001, LY15B050001, S16H180002); Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province (Nos. 2014006, 2014007); Open Foundation of the Key Laboratory of Clay Minerals, the Ministry of Land and Resources of China (No. 2014-K03).

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120

被引次数

6

摘要：

建立了一种离子色谱-柱切换-安培检测技术同步测定饮料中三氯蔗糖、葡萄糖、果糖和蔗糖的方法。以CarboPac PA10阴离子交换保护柱和分析柱进行分离，用水和250 mmol/L NaOH梯度淋洗，脉冲安培检测。弱保留糖类在25 mmol/L NaOH流动相淋洗下分离检测，通过柱切换将保护柱切换至分析柱后，同时切换至250 mmol/L NaOH淋洗，三氯蔗糖仅通过较短的保护柱分离，4种糖类能够得到同步检测。4种糖类在0.01~20 mg/L范围内有良好的线性关系和较低的检出限，重复性好，样品测定的回收率分别为90.38%~102.88%（三氯蔗糖）、95.56%~102.75%（葡萄糖）、101.66%~114.33%（果糖）和105.03%~106.49%（蔗糖）。该方法可广泛应用于食品中强保留物质的测定。

关键词: 离子色谱, 三氯蔗糖, 柱切换

Abstract:

A new method of simultaneous determination of sucralose, glucose, fructose and sucrose in beverage has been established using ion-exchange chromatography coupled with column-switching and pulsed amperometric detection. A CarboPac PA10 guard column and a CarboPac PA10 analytical column, along with the mixtures of pure water and 250 mmol/L NaOH solution in different ratio acting as mobile phase in the gradient elution were used in the pulsed amperometric detection. After the separation and detection of saccharides with weak retention through elusion with 25 mmol/L NaOH, we switched the guard column to analytical column, in which sucralose can be separated through the comparatively short guard column while the four saccharides can be detected simultaneously. In this study, four saccharides had good linear relationships in 0.01-20 mg/L and the recoveries were 90.38%-102.88% (sucralose), 95.56%-102.75% (glucose), 101.66%-114.33% (fructose), 105.03%-106.49% (sucrose) with high sensitivity and good reproducibility. This method can be widely used in the determination of components with strong retention in food.

Key words: column-switching, ion-exchange chromatography, sucralose

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