基于微流控芯片技术的秀丽隐杆线虫研究进展

doi:10.3724/SP.J.1123.2016.07017

摘要/Abstract

摘要：

秀丽隐杆线虫具有体积小、生命周期短、结构简单和高基因保守性等特点，是生命科学研究领域中的一种重要模式生物。微流控芯片的通道尺寸与线虫大小相匹配，并可实现灵活集成的线虫操控，为线虫研究提供了一种全新的平台。在微流控平台上，线虫长期培养、固定、分选、精确刺激传递和单线虫包裹等单元操作已经实现，并被应用于线虫神经生物学、行为学、衰老及发育、药物筛选等研究中。该文着重介绍近几年基于微流控芯片技术的线虫研究最新进展，并对其应用前景予以展望。

关键词: 微流控芯片, 秀丽隐杆线虫, 液滴, 综述

Abstract:

The nematode Caenorhabditis elegans (C. elegans) has been widely used as a multicellular organism for biological research due to its simplicity, short life cycle, and relevance to human genetics and biology. In the past decade, microfluidics technology was emerging as a promising platform for the study of C. elegans both in continuous flow-based or droplet-based format. Worm long-term culture, immobilization, sorting, stimuli exposure and individual worm encapsulation have been realized on microfluidic platform. By providing unique capabilities with precise manipulations and controlled microenvironment, this platform has been used for the study of neurobiology, behaviors, aging and development, and drug screening in C. elegans. In this review, we highlight the recent advances of microfluidic technology in worm manipulations and its biomedical applications. The prospects and challenges of microfluidic technology for future studies are discussed as well.

Key words: Caenorhabditis elegans (C. elegans), droplets, microfluidics, review

中图分类号:

O658

尹方超, 温慧, 朱国丽, 秦建华. 基于微流控芯片技术的秀丽隐杆线虫研究进展[J]. 色谱, 2016, 34(11): 1031-1042.

YIN Fangchao, WEN Hui, ZHU Guoli, QIN Jianhua. Advances of Caenorhabditis elegans research on microfluidic device[J]. Chinese Journal of Chromatography, 2016, 34(11): 1031-1042.

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