色谱

色谱 ›› 2016, Vol. 34 ›› Issue (10): 960-963.DOI: 10.3724/SP.J.1123.2016.07033

• 特别策划:离子态化合物色谱分析专栏 • 上一篇    下一篇

阀切换-离子色谱法测定难溶性药物中的氯离子和三氟乙酸根

金菊花1, 叶明立2   

  1. 1. 浙江省立同德医院, 浙江 杭州 310028;
    2. 浙江大学化学系, 浙江 杭州 310028
  • 收稿日期:2016-07-26 出版日期:2016-10-08 发布日期:2013-04-03
  • 通讯作者: 金菊花

Determination of chloride and trifluoroacetate in water- insoluble pharmaceuticals by valve switch-ion chromatography

JIN Juhua1, YE Mingli2   

  1. 1. Tongde Hospital of Zhejiang Province, Hangzhou 310028, China;
    2. Chemistry Department of Zhejiang University, Hangzhou 310028, China
  • Received:2016-07-26 Online:2016-10-08 Published:2013-04-03

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摘要：

建立了头孢克肟、月桂酰吲达帕胺、布地奈德等难溶性药物中氯离子和三氟乙酸根的阀切换-离子色谱检测方法。分离柱为IonPac AS23柱，通过考察阴离子保留情况，选择3.5 mmol/L碳酸钠+1.0 mmol/L碳酸氢钠作淋洗液，对难溶性药物中氯离子和三氟乙酸根进行了分析。在上述条件下，氯离子和三氟乙酸根能很好地分离，并在质量浓度为0.10~10.00 mg/L时与对应色谱峰面积之间的线性关系良好，检出限分别为0.006 mg/L和0.06 mg/L，回收率为90%~94%。该方法简便、快速、准确，可用于实际样品的测定。

关键词: 离子色谱, 氯离子, 难溶性药物, 三氟乙酸根

Abstract:

A method was developed for the determination of chloride and trifluoroacetate in water-insoluble pharmaceuticals such as cefixime, lauroyl-indapamide, budesonide by valve switch-ion chromatography. Chromatographic separation was performed on an IonPac AS23 column, 3.5 mmol/L sodium carbonate and 1.0 mmol/L sodium bicarbonate eluents were used. The effects of the types of column and eluent, eluent concentration of inorganic anions were investigated. The linear ranges of the method for seven anions were 0.10-10.00 mg/L, and the detection limits (S/N=3) of chloride and trifluoroacetate were 0.006 mg/L and 0.06 mg/L respectively. The recoveries of inorganic anions spiked in cefixime were between 90% and 94%. The method was successfully used in the determination of inorganic anions in real samples.

Key words: chloride, ion chromatography (IC), trifluoroacetate, water-insoluble pharmaceuticals

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