色谱

色谱 ›› 2017, Vol. 35 ›› Issue (8): 855-859.DOI: 10.3724/SP.J.1123.2017.03001

• 研究论文 • 上一篇    下一篇

非对称场流分离技术在蛋黄浆质低密度脂蛋白粒径表征中的应用

张文惠1,2, 蔡春雪1, 王静2, 毛震1, 李月秋3, 丁良1, 申世刚2, 窦海洋1,2   

  1. 1. 河北大学医学院, 河北 保定 071000;
    2. 河北大学化学与环境科学学院, 河北 保定 071002;
    3. 河北大学医学实验中心, 河北 保定 071000
  • 收稿日期:2017-03-01 出版日期:2017-08-08 发布日期:2013-11-25
  • 通讯作者: 丁良,Tel:5075660,E-mail:345823685@qq.com;窦海洋,Tel:5078525,E-mail:douhaiyang-1984@163.com
  • 基金资助:

    河北省自然科学基金项目（B2016201002）；河北省引进留学人员资助项目（CL201603）；河北省高等学校科技研究基金项目（QN2016081）；河北省医学学科研究重点课题（20160048）；保定市科学研究与发展计划项目（15ZG049）.

Application of asymmetrical flow field-flow fractionation for size characterization of low density lipoprotein in egg yolk plasma

ZHANG Wenhui1,2, CAI Chunxue1, WANG Jing2, MAO Zhen1, LI Yueqiu3, DING Liang1, SHEN Shigang2, DOU Haiyang1,2   

  1. 1. College of Medicine, Hebei University, Baoding 071000, China;
    2. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
    3. Medical Experimental Center of Hebei University, Baoding 071000, China
  • Received:2017-03-01 Online:2017-08-08 Published:2013-11-25
  • Supported by:

    Natural Science Foundation of Hebei Province (No. B2016201002); Hebei Introduction Foundation for the Returned Overseas Scholars (No. CL201603); Research Project of Science and Technique of Universities in Hebei Province (No. QN2016081); Important Project of Medicine Science and Research of Hebei Province (No. 20160048); Project of Science Research and Development Plan of Baoding (No. 15ZG049).

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摘要：

通过自组装的非对称场流分离系统（AF4）与紫外可见光检测器联用分离表征了笼养鸡蛋、柴鸡蛋、鹌鹑蛋和鸭蛋蛋黄浆质中的低密度脂蛋白（LDL）。在近似蛋黄浆质生理条件下，研究了进样量、交叉流流速、膜的类型对AF4蛋黄浆质中LDL分离表征的影响；考察了该方法的精密度。在优化的AF4分析条件下，检测出了笼养鸡蛋、柴鸡蛋、鹌鹑蛋和鸭蛋蛋黄浆质中LDL的水力学粒径分布。LDL的AF4洗脱峰高和峰面积的日内精密度分别为1.3%和1.9%（n=7），日间精密度分别为2.4%和2.3%（n=7）。研究结果表明，该方法可用于分离禽类蛋黄浆质中的LDL，同时能够得到LDL水力学粒径分布。

关键词: 蛋黄浆质, 低密度脂蛋白, 非对称场流分离, 粒径表征

Abstract:

Home-made asymmetrical flow field-flow fractionation (AF4) system, online coupled with ultraviolet/visible (UV/Vis) detector was employed for the separation and size characterization of low density lipoprotein (LDL) in egg yolk plasma. At close to natural condition of egg yolk, the effects of cross flow rate, sample loading, and type of membrane on the size distribution of LDL were investigated. Under the optimal operation conditions, AF4-UV/Vis provides the size distribution of LDL. Moreover, the precision of AF4-UV/Vis method proposed in this work for the analysis of LDL in egg yolk plasma was evaluated. The intra-day precisions were 1.3% and 1.9% (n=7) and the inter-day precisions were 2.4% and 2.3% (n=7) for the elution peak height and elution peak area of LDL, respectively. Results reveal that AF4-UV/Vis is a useful tool for the separation and size characterization of LDL in egg yolk plasma.

Key words: asymmetrical flow field-flow fractionation (AF4), egg yolk plasma, low density lipoprotein (LDL), size characterization

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