非对称流场流分离技术的现状及发展趋势

doi:10.3724/SP.J.1123.2017.05021

摘要/Abstract

摘要：

场流分离是生物分析领域一项成熟的技术，将流体与外场联合作用于待分离物质，利用分析物某些理化参数上的差异进行分离。非对称流场流是其重要的分支之一，所施加的外力场为垂直方向的液流，分离过程于开放型的通道中在某种组成的载液迁移推动下进行，主要根据分析物与垂直施加的第二维液流之间的相互作用完成分离。非对称流场流在蛋白质、蛋白质复合物、衍生纳米级/微米级粒子、亚细胞单元和聚合物等分离中的应用日益广泛，主要归功于其直接应用于生物样品时可进行无损分离，因此生物分析物如蛋白质可以在生物友好型的环境中完成分离而不改变其构型，也无需使用降解载液。分离设备便于保持无菌状态，分析物可在生物友好的环境中维持其自然状态。该文简要描述了场流分离原理并罗列出其在生物分析领域一些卓越的发展和应用。

关键词: 表征, 非对称流场流分离, 分离, 生物分析, 综述

Abstract:

Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field). Asymmetrical flow field-flow fractionation (AF4) is a vital subvariant of FFF, which applying a vertical flow field as the second dimension force field. The separation in AF4 opening channel is carried out by any composition carrier fluid, universally and effectively used in separation of bioparticles and biopolymers due to the non-invasivity feature. Herein, bio-analytes are held in bio-friendly environment and easily sterilized without using degrading carrier fluid which is conducive to maintain natural conformation. In this review, FFF and AF4 principles are briefly described, and some classical and emerging applications and developments in the bioanalytical fields are concisely introduced and tabled. Also, special focus is given to the hyphenation of AF4 with highly specific, sensitive detection technologies.

Key words: asymmetrical flow field-flow fractionation (AF4), bioanalysis, characterization, review, separation

中图分类号:

O658

梁启慧, 吴迪, 邱百灵, 韩南银. 非对称流场流分离技术的现状及发展趋势[J]. 色谱, 2017, 35(9): 918-926.

LIANG Qihui, WU Di, QIU Bailing, HAN Nanyin. Present situation and development trends of asymmetrical flow field-flow fractionation[J]. Chinese Journal of Chromatography, 2017, 35(9): 918-926.

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