香水中邻苯二甲酸酯总量测定及其暴露评估

doi:10.3724/SP.J.1123.2017.08026

摘要/Abstract

摘要：

建立了快速测定香水中邻苯二甲酸酯（PAEs）的前处理方法。将香水中的PAEs水解为邻苯二甲酸（PA），再利用磷酸三丁酯萃取酸化液中的PA，使用高效液相色谱-二极管阵列检测器定量分析，并评估了PAEs的暴露量。实验考察并优化了影响PAEs水解率的因素，包括KOH的浓度和体积、乙醇的体积、水解时间和温度，优化后的水解条件：10 mL 4 mol/L KOH，1 mL乙醇，水解时间20 min，水解温度80℃。目标分析物的线性范围为3~240 μmol/L（R²=0.9991），LOD和LOQ分别为4.6 μmol/kg和5.9 μmol/kg，加标回收率为83.4%~92.7%，日内和日间精密度（RSD）不大于6.8%（n=5）。选择35种香水进行测定，PAEs总量范围在

关键词: 暴露水平, 高效液相色谱, 邻苯二甲酸, 水解, 香水

Abstract:

A novel method for rapid screening of phthalates (PAEs) in perfumes was developed. The PAEs were hydrolyzed to phthalic acid (PA), and the PA in the acidified solution was extracted with tributyl phosphate (TBP) which was detected by high performance liquid chromatography-diode array detection (HPLC-DAD). Meanwhile exposure dose to PAEs was estimated by the percentage of a topically applied dose that permeates the skin. The parameters such as the concentration and volume of KOH, the volume of ethanol, hydrolysis time and temperature were employed to evaluate the hydrolysis efficiency of PAEs. The optimized hydrolysis conditions were 10 mL of 4 mol/L KOH, and 1 mL of ethanol at 80℃ for 20 min. The linear range of phthalic acid was 3-240 μmol/L with a good correlation coefficient (R²=0.9991). The limits of detection (LOD) and quantification (LOQ) were 4.6 μmol/kg and 5.9 μmol/kg, respectively. The recoveries varied from 83.4% to 92.7% with relative standard deviations equal to or lower than 6.8%(n=5). A total of 35 perfume samples were determined, and the contents of total PAEs were found in the range of

Key words: exposure dose, high performance liquid chromatography (HPLC), hydrolysis, perfumes, phthalates (PAEs)

中图分类号:

O658

赵斯含, 王正萌, 邓红侠, 段家惠, 王进义, 刘书慧. 香水中邻苯二甲酸酯总量测定及其暴露评估[J]. 色谱, 2017, 35(12): 1301-1305.

ZHAO Sihan, WANG Zhengmeng, DENG Hongxia, DUAN Jiahui, WANG Jinyi, LIU Shuhui. Determination of total phthalates in perfume and their exposure assessment[J]. Chinese Journal of Chromatography, 2017, 35(12): 1301-1305.

参考文献

[1] Li X M, Wang J, Zhang Q H, et al. Chinese Journal of Chromatography, 2015, 33(11):1147李晓敏, 王景, 张庆合, 等. 色谱, 2015, 33(11):1147
[2] Hubinger J C. J Cosmet Sci, 2010, 61(6):457
[3] Kwapniewski R, Kozaczka S, Hauser R, et al. J Occup Environ Med, 2008, 50(6):705
[4] Duty S M, Ackerman R M, Calafat A M, et al. Environ Health Perspect, 2005, 113(11):1530
[5] Shen H Y, Jiang H L, Mao H L, et al. J Sep Sci, 2007, 30(1):48
[6] Koniecki D, Wang R, Moody R P, et al. Environ Res, 2011, 111(3):329
[7] Guo Y, Kannan K. Environ Sci Technol, 2013, 47(24):14442
[8] Bao J, Wang M, Ning X, et al. J Toxicol Environ Health A, 2015, 78(5):325
[9] Ministry of Health of the People's Republic of China. Hygienic Standard for Cosmetics. (2008-07-01). http://www.maya777.com/show.aspx?cid=41&id=96中华人民共和国卫生部. 化妆品卫生规范. (2008-07-01). http://www.maya777.com/show.aspx?cid=41&id=96
[10] Regulation (EC) No 1223/2009 of the European Parllament and of the Council of 30 November 2009 on Cosmetic Products. Official Journal of the European Union. (2009-12-22). http://www.doc88.com/p-77683557145.html
[11] Su R, Zhao X, Li Z, et al. Anal Chim Acta, 2010, 676(1):103
[12] Luo Y B, Yu Q W, Yuan B F, et al. Talanta, 2012, 90(1):123
[13] Liu S, Liu L, Han Y, et al. J Chromatogr A, 2015, 1420:26
[14] Ostrovsky I, Cabala R, Kubinec R, et al. Food Chem, 2011, 124(1):392
[15] Hubinger J C, Havery D C. J Cosmet Sci, 2006, 57(2):127
[16] Koo H J, Lee B M. J Toxicol Environ Health A, 2004, 67(23/24):1901
[17] Alsaleh I, Elkhatib R. Environ Sci Pollut Res Int, 2016, 23(1):455
[18] Barreca S. J Toxicol Environ Health A, 2015, 78(15):1008
[19] Bao J Q, Wang M, Gao X, et al. Food Science, 2014, 35(23):337鲍佳沁, 王敏, 高曦, 等. 食品科学, 2014, 35(23):337
[20] Wormuth M, Scheringer M, Vollenweider M, et al. Risk Anal, 2006, 26(3):803
[21] United States Environmental Protection Agency. Child-Specific Exposure Factors Handbook. EPA/600/R-06/096F. (2008-08-20). http://cfpub.epa.gov/ncea/CFM/SU.S
[22] Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers. Opinion on Diethyl Phthalate in Cosmetic Products Adopted During the 20th Plenary Meeting of 4 June 2002, Brussels. (2002-06-04). http://www.doc88.com/p-7364584093634.html
[23] Health Canada. Exposure Factors for Assessing Total Daily Intake of Priority Substances by the General Population of Canada. Ottawa (ON) Priority Substances Section, Environmental Health Directorate. (1998-12-11). http://www.tandfonline.com/doi/ref/10.1080/10937404.2013.769419?scroll=top&

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