色谱

色谱

• “多维色谱”专栏 • 上一篇    下一篇

复杂生物体系中蛋白质高效分离分析技术的新进展

孟庆芳,张养军,王京兰，蔡耘,钱小红   

  1. Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China
  • 收稿日期:2004-06-21 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 钱小红
  • 基金资助:
    国家自然科学基金(项目号:20275046,20405017)、国家科技攻关计划项目(项目号:2202-BA711A11)及北京市科技计划项目(课题编号:H030230280190)资助.

New Advances of High Performance Separation and Analytical Techniques for Proteins in Complex Biological Systems

MENG Qingfang, ZHANG Yangjun, WANG Jinglan, CAI Yun, QIAN Xiaohong   

  1. Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China
  • Received:2004-06-21 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

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摘要： 继人类基因组计划完成之后,作为一种新的研究策略,蛋白质组学在生命科学研究中发挥着愈来愈重要的作用。由于生物体系的复杂性和多样性,使得分离效率高、灵敏度高、通量高和动态范围宽的分离分析技术平台的研究和应用已成为蛋白质组学研究的重点和热点之一。着重介绍了近年来应用日益广泛的多维色谱预分离、毛细管液相色谱-质谱联用、毛细管电泳及其与质谱联用等高效分离分析技术在复杂生物体系的蛋白质分析中的最新进展。引用相关文献40篇。

关键词: 蛋白质组学, 多维色谱, 复杂生物体系 , 毛细管电泳-质谱, 毛细管液相色谱-质谱

Abstract: As a new strategy, proteomics is playing more and more important roles in the life sciences after the sequencing of the human genome was completed. Because a biological system is very complex and diverse, the study and application of technological platform for the separation and analysis with high resolution, high sensitivity, high throughput and broad dynamic range have become one of the important and hot spots. The review focused on the new progress of high performance separation and analysis techniques which were applied to the field of proteomics increasingly, such as multidimensional liquid chromatography for pre-fractionation, capillary liquid chromatography coupled with mass spectrometry and capillary electrophoresis interfaced on line with mass spectrometry.

Key words: capillary electrophoresis interfaced with mass spectrometry, capillary liquid chromatography coupled with mass spectrometry, complex biological system , multidimensional chromatography, proteomics