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无死体积蚀刻多孔膜接口的制作及其在二维毛细管电泳分离系统中的应用

刘和春,张丽华,张维冰,张玉奎   

  1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China
  • 收稿日期:2004-06-30 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 张玉奎
  • 基金资助:

Fabrication of an Etched Porous Interface and Its Application in Capillary Electrophoresis-Based

LIU Hechun, ZHANG Lihua, ZHANG Weibing, ZHANG Yukui   

  1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China
  • Received:2004-06-30 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

717

被引次数

13

摘要： 介绍了一种在毛细管柱上原位腐蚀而成的多孔膜接口的制作方法,并用该接口构建了一类毛细管电泳二维分离技术平台。柱上原位腐蚀刻成的多孔膜接口具有零死体积、制作过程简易、成本低廉、耐用、柱间切换便捷等优点,特别适合作为基于毛细管柱的二维及多维电泳联用中的接口,是目前二维及多维毛细管柱联用中一类较为新型、实用、理想的接口。以鹿茸冻干粉可溶物样品为例,验证了该接口在二维毛细管电泳联用系统中的可行性和分离效能。实验结果表明：鹿茸冻干粉可溶物整个二维分离分析的时间在1 h内完成,二维分离系统的分辨率和总峰容量都比一维的高。

关键词: 二维分离平台 , 毛细管电泳, 原位腐蚀多孔石英膜接口

Abstract: An on-column, etched fused-silica porous junction interface was designed and fabricated, and an integrated two-dimensional separation platform involving on-line coupling of capillary isoelectric focusing (CIEF) with capillary electrophoresis (CE) was constructed by the interface. The primary advantages of this novel porous junction interface over previous designs are no dead volume, simplicity, ruggedness and convenience of column switching. This interface is particularly well suited for on-line coupling of CE-based multiple dimensional separation system. The feasibility and performance of the 2D CIEF-CE system constructed by such an etched porous junction were evaluated by the separation of soluble extracts of an antler. The results indicated that the total separation time was less than 1 h, and the 2D CE separation system was found to increase the resolving power and overall peak capacity over single dimension mode.

Key words: capillary electrophoresis, two-dimensional separation system , etched fused-silica porous junction interface