色谱

色谱

• 研究简报 • 上一篇    下一篇

反相高效液相色谱法测定不同产地白花蛇舌草中的对香豆酸

张才华,郭兴杰,宝炉丹,秦峰,李发美   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2004-03-25 修回日期:1900-01-01 出版日期:2005-03-30 发布日期:1988-12-25
  • 通讯作者: 李发美
  • 基金资助:

Determination of p-Coumaric Acid in Hedyotis diffusa Willd. from Different Sources by Reversed-Phase High Performance Liquid Chromatography

ZHANG Caihua, GUO Xingjie, BAO Ludan, QIN Feng, LI Famei   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2004-03-25 Revised:1900-01-01 Online:2005-03-30 Published:1988-12-25

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713

被引次数

13

摘要： 用反相高效液相色谱法对不同产地的白花蛇舌草中对香豆酸的含量进行了测定。色谱柱采用DiamonsilTM ODS柱(4.6 mm i.d.×250 mm, 5 μm),流动相为乙腈-20 mmol/L NH4Ac(pH 4.0)(体积比为15∶85),流速1.0 mL/min,柱温为室温,检测波长为308 nm。在上述条件下测得对香豆酸的质量浓度为4.04～202 mg/L时与色谱峰面积之间线性关系良好(r＝0.9996),高中低浓度条件下的平均回收率为97.4％~102.2%。该方法快速简便,精密度高,重现性和准确度良好,可以作为白花蛇舌草质量控制的一个有效方法。

关键词: 白花蛇舌草, 对香豆酸, 反相高效液相色谱法, 中药材

Abstract: A method for the determination of p-coumaric acid in Hedyotis diffusa Willd. from different sources by reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. p-Coumaric acid was successfully separated on a DiamonsilTM ODS column (4.6 mm i.d.×250 mm, 5 μm) at ambient temperature, using a mixture of CH3CN-20 mmol/L NH4Ac (pH 4.0) (15∶85, v/v) as mobile phase and detection at 308 nm. The flow rate was 1.0 mL/min. There was good linear relationship (r=0.9996) between the mass concentration and the peak area of p-coumaric acid in the range of 4.04-202 mg/L. The recoveries were found to be in the range of 97.4%-102.2%. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility. The method is suitable for use in quality control of Hedyotis diffusa Willd. from different sources.

Key words: Chinese medical material , Hedyotis diffusa Willd., p-coumaric acid, reversed-phase high performance liquid chromatography