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色谱

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毛细管电泳-电化学检测法检测水解植物蛋白调味液中的3-氯-1,2-丙二醇及其应用

邢晓平1,2,曹玉华1

  

  1. 1.School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China; 2.Department of Science and Industry, Yancheng Institute of Technology, Yancheng 224003, China
  • 收稿日期:2005-04-08 修回日期:2005-11-16 出版日期:2006-03-30 发布日期:1987-06-25
  • 通讯作者: 曹玉华

Study on Elimination of 3-Chloro-1,2-Propanediol in Hydrolyzed Vegetable Protein by Capillary Electrophoresis
with Electrochemical Detection

XING Xiaoping1,2, CAO Yuhua1

  

  1. 1.School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China; 2.Department of Science and Industry, Yancheng Institute of Technology, Yancheng 224003, China
  • Received:2005-04-08 Revised:2005-11-16 Online:2006-03-30 Published:1987-06-25
  • Contact: CAO Yuhua

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摘要：

运用毛细管电泳-电化学检测法研究了水解植物蛋白液中3-氯-1,2-丙二醇(3-MCPD)含量的测定。探索了3-MCPD检测的最佳电泳条件。以328 μm的铜圆盘电极为工作电极,在电极电位为+0.65 V(参比电极为饱和甘汞电极),检测池缓冲液为0.05 mol/L氢氧化钠,运行缓冲液为30 mmol/L硼砂(pH 9.24)时,3-MCPD能被很好地分离。3-MCPD的线性范围为6.6~200 mg/L,检测限为0.22 mg/L。研究了水解缓冲液的pH值、水解温度、水解时间等因素对3-MCPD水解反应的影响,用毛细管电泳-电化学检测法检测水解反应过程中3-MCPD的含量。结果表明,在pH 8.0的水解缓冲液中,将水解植物蛋白调味液前体于90 ℃条件下恒温加热1 h，能有效地控制3-MCPD的含量在1.0 mg/kg以下,从而达到我国食品安全允许的限量标准。

关键词: 3-氯-1,2-丙二醇, 电化学检测, 毛细管电泳, 水解反应
, 水解植物蛋白调味液

Abstract:

A method was developed to estimate 3-chloro-1,2-propanediol (3-MCPD) in hydrolyzed vegetable protein (HVP) by capillary electrophoresis with electrochemical detection. The optimum conditions were investigated to determine 3-MCPD in hydrolyzed vegetable protein. In 30 mmol/L borax (pH 9.24) running buffer, separation of 3-MCPD from glycerol was achieved. A 328 μm copper-disk electrode used as the working electrode exhibited a good response at +0.65 V (vs. saturated calomel electrode (SCE)) in 0.05 mol/L sodium hydroxide solution. The effects of pH value, temperature and hydrolysis time on the elimination of 3-MCPD in hydrolyzed vegetable protein were studied. By adjusting hydrolyzed vegetable protein to pH value of 8.0 and heating up for 1 h at 90 ℃, the content of 3-MCPD in hydrolyzed vegetable protein could be controlled under the level of 1.0 mg/kg, which coincides with the criterion of food safety in China.

Key words: 3-chloro-1,2-propanediol (3-MCPD), electrochemical detection, hydrolysis , hydrolyzed vegetable protein (HVP), capillary electrophoresis (CE)