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色谱

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毛细管电泳法同时测定血清中的左旋多巴和甲基多巴

王春1,王志1,韩丹丹2,胡彦学2,赵锦2,杨秀敏2,宋双居1
   

  1. 1.College of Science, Agricultural University of Hebei, Baoding 071001, China; 2.College of
    Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China

  • 收稿日期:2005-08-18 修回日期:2005-10-27 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 王 志

Simultaneous Determination of Levodopa and Methyldopa in Human Serum by Capillary Electrophoresis

WANG Chun1, WANG Zhi1, HAN Dandan2, HU Yanxue2, ZHAO Jin2,YANG Xiumin2, SONG Shuangju1

  

  1. 1.College of Science, Agricultural University of Hebei, Baoding 071001, China; 2.College of
    Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China

  • Received:2005-08-18 Revised:2005-10-27 Online:2006-07-30 Published:1986-12-25
  • Contact: Wang Zhi

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摘要：

建立了毛细管电泳-紫外检测同时测定血清中左旋多巴和甲基多巴的方法。以40 mmol/L硼砂（pH 9.5）为分离缓
冲溶液,在3.45 kPa（0.5 psi）压力下进样7 s、分离电压22 kV、检测波长200 nm、温度25 ℃的条件下进行测定,两
种物质获得了较好的分离。甲基多巴和左旋多巴分别为1.0~64.0 mg/L和1.0~71.0 mg/L时与峰面积呈良好的线性关系,
线性相关系数分别为0.9998和0.9994,检出限分别为0.6和0.8 mg/L(以信噪比为3计)。将该法用于血清中甲基多巴和左
旋多巴的测定,回收率为82.8%~88.8%,相对标准偏差为2.10%~2.63%。

关键词: 甲基多巴, 毛细管电泳法, 血清 , 左旋多巴

Abstract:

A simple capillary electrophoresis method was developed for the determination of levodopa
and methyldopa in human serum. The effects of pH and concentration of buffer, voltage and injection
time on separation were investigated. As a result, an optimized separation was obtained with a
fused-silica capillary of 60.2 cm (50 cm effective length)×75 μm i.d. in a running buffer of 40
mmol/L sodium tetraborate (pH 9.5) with an applied voltage of 22 kV at 25 ℃. Sample introduction
was performed at 3.45 kPa （0.5 psi） for 7 s and on-column detection was made with a diode array
detector at 200 nm. The linear responses covered the ranges from 1.0 to 64.0 mg/L (r=0.9998) for
methyldopa and from 1.0 to 71.0 mg/L (r=0.9994) for levodopa. The detection limits (S/N=3) of
methyldopa and levodopa were shown to be 0.6 mg/L and 0.8 mg/L, respectively. The recoveries for
levodopa and methyldopa in human serum were between 82.8% and 88.8% with relative standard
deviations between 2.10% and 2.63%.

Key words: serum , levodopa, methyldopa, capillary electrophoresis