色谱

色谱

• 研究论文 • 上一篇    下一篇

槲寄生中高圣草素-7-O-β-D-葡萄糖苷的分离及含量测定

赵云丽,马铭研,高晓霞,刘涛,于治国,毕开顺

  

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2005-10-12 修回日期:2006-04-12 出版日期:2006-09-30 发布日期:1986-09-25
  • 通讯作者: 毕开顺

Isolation and Determination of Homoeriodictyol-7-O-β-D-Glycoside in Viscum coloratum

ZHAO Yunli, MA Mingyan, GAO Xiaoxia, LIU Tao, YU Zhiguo, BI Kaishun   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2005-10-12 Revised:2006-04-12 Online:2006-09-30 Published:1986-09-25
  • Contact: BI Kaishun

PDF

579

被引次数

12

摘要： 对槲寄生中的有效成分高圣草素-7-O-β-D-葡萄糖苷进行了分离和结构鉴定,并对其含量进行了分析。色谱条件：C18柱(200 mm×4.6 mm i.d., 5 μm),流动相为乙腈-0.5%冰醋酸水溶液(体积比为18∶82),流速为1.0 mL/min，柱温为30 ℃,检测波长为284 nm,进样量为10 μL。结果表明,高圣草素-7-O-β-D-葡萄糖苷的峰面积与其质量浓度有良好的线性关系，相关系数r为0.9997；方法的加标回收率为96.0%~100.1%。该方法简便、快速、准确,精密度好，可作为槲寄生质量控制的一个有效方法。

关键词: 反相高效液相色谱法, 高圣草素-7-O-β-D-葡萄糖苷, 槲寄生

Abstract: The homoeriodictyol-7-O-β-D-glycoside was isolated from Viscum coloratum and identified by mass spectrometry and nuclear magnetic resonance (NMR) (1H NMR and 13C NMR). A method for determination of homoeriodictyol-7-O-β-D-glycoside in Viscum coloratum was developed by using a Kromasil C18 column (200 mm×4.6 mm i.d., 5 μm) with a mixture of acetonitrile and 0.5% glacial acetic acid solution (18∶82, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 284 nm and temperature was set at 30 ℃. The volume of injection was 10 μL. Good linear relationship (r=0.9997) between the mass concentration and the peak area of homoeriodictyol-7-O-β-D-glycoside was obtained in the range of 1.0-32.0 mg/L. The recoveries were found to be in the range of 96.0%-100.1%. The results of the experiments demonstrated that the established method is rapid and simple with good accuracy and reproducibility. The method is suitable for the quality control of Viscum coloratum from different sources.

Key words: homoeriodictyol-7-O-β-D-glycoside, Viscum coloratum , reversed-phase high performance liquid chromatography (RP-HPLC)