色谱

色谱

• 研究论文 • 上一篇    下一篇

配位色谱法从葛根素浸膏中分离纯化葛根素

潘见 袁传勋 戴郁青   

  1. Engineering Research Center of Bio-Process of Ministry of Education, Hefei University of Technology, Hefei 230069, China
  • 收稿日期:2005-10-12 修回日期:2006-01-06 出版日期:2006-09-30 发布日期:1986-09-25
  • 通讯作者: 袁传勋

Isolation and Purification of Puerarin from Puerarin Extractive by Chelate Complex Chromatography

PAN Jian, YUAN Chuanxun, DAI Yuqing   

  1. Engineering Research Center of Bio-Process of Ministry of Education, Hefei University of Technology, Hefei 230069, China
  • Received:2005-10-12 Revised:2006-01-06 Online:2006-09-30 Published:1986-09-25
  • Contact: YUAN Chuanxun

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摘要： 建立了采用配位色谱柱从葛根素浸膏中分离纯化葛根素的方法。以铜离子为中心离子,制备了中心离子含量为7%的配位色谱柱。样品上样于配位色谱柱后，以氯仿-甲醇（体积比为10∶1）混合溶剂洗脱，得到了较纯的葛根素,较之用传统的硅胶色谱柱纯化，纯度提高了11%,回收率提高了12%,且柱容量提高了两倍。配位色谱改变了葛根素在传统硅胶柱上的洗脱顺序,对目标物质的分辨率比传统硅胶色谱柱高。

关键词: 分离纯化, 葛根素, 葛根素浸膏 , 配位色谱, 铜离子

Abstract: Puerarin, an isoflavone compound, is an important ingredient in traditional Chinese medicine, which has the good medical effects on hypertension and angina. A new type of separation and purification method for isolating puerarin was developed using chelate complex chromatography. The stationary phase was prepared with silica gel containing 7% of Cu(OAc)2 in the chloroform. After the chloroform was evaporated, it was used as chelate complex chromatographic stationary phase to purify puerarin extractive. Pure puerarin was obtained when a mixture of chloroform and methanol (10∶1, v/v) was used as eluent. In comparison with a common silica gel column, the chelate complex chromatographic column was more convenient and efficient. The purity and recovery of puerarin, and column capacity were improved 11%, 12% and 200%, respectively. Under the optimized experimental conditions， the purity of puerarin could be as high as 93％with a recovery of 92%.

Key words: copper ion, isolation and purification, puerarin, puerarin extractive , chelate complex chromatography