色谱

色谱

• 兴奋剂检测方法专栏 • 上一篇    下一篇

毛细管区带电泳用于多种类兴奋剂的同时快速分离检测

肖惠,童萍,冯强,张兰   

  1. Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education, Sport Science Research Center, Fuzhou University, Fuzhou 350002, China
  • 收稿日期:2008-01-05 修回日期:2008-07-30 出版日期:2008-07-30 发布日期:1983-12-25
  • 通讯作者: 张兰
  • 基金资助:
    福建省体育局重大专项（HX2005-74）、福建省自然科学基金(B0510006, 2007J0219)、福建省科技厅国际合作重点项目(2007I0020)和福建省高校新世纪优秀人才支持计划资助.

A rapid capillary zone electrophoresis method for simultaneous determination of several kinds of doping agents

XIAO Hui, TONG Ping, FENG Qiang, ZHANG Lan   

  1. Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education, Sport Science Research Center, Fuzhou University, Fuzhou 350002, China
  • Received:2008-01-05 Revised:2008-07-30 Online:2008-07-30 Published:1983-12-25
  • Contact: ZHANG Lan

PDF

1122

被引次数

7

摘要： 建立了一种同时分离检测包括利尿剂、蛋白同化剂、β-阻断剂、麻醉剂、β2-激动剂、刺激剂等6类8种兴奋剂的毛细管区带电泳-紫外检测法。优化的色谱条件为：以50 mmol/L甲酸铵-氨水(pH 7.8)缓冲液为运行液,于3 kPa下进样10 s,分离电压为20 kV,检测波长为214 nm。在此条件下，8种兴奋剂在7 min内实现了快速的基线分离。在相应的浓度范围内,8种组分的浓度与峰高呈良好的线性关系,检出限达为0.2~0.7 μg/mL。该方法快速，分析成本低，无污染,非常适用于多种类兴奋剂的同时快速检测。

关键词: 多种类兴奋剂, 毛细管区带电泳, 同时检测

Abstract: A capillary zone electrophoresis-ultraviolet detection (CZE-UV) method was established for the simultaneous determination of eight doping agents which belong to diuretics, anabolic steroids, β-blocks, narcotics, β2-agonists and stimulants. Under the optimal conditions, with 50 mmol/L ammonium formate buffer (pH 7.8) as the running buffer, injection at a pressure of 3 kPa for 10 s, applied voltage of 20 kV, and detection at 214 nm, eight doping agents were separated quickly and completely within 7 min. The linearities of the eight doping anents were good in respective concentration ranges. Their detection limits were from 0.2 to 0.7 μg/mL. It is a simple and rapid method with small sample size for doping detection.

Key words: several kinds of doping agents, simultaneous determination , capillary zone electrophoresis （CZE）