色谱

色谱

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云南植物--露水草中的一种新植物甾酮的分离与分析

谭成玉1*, 孔亮1, 李铣2, 李伟1, 李宁2   

  1. 1. 大连海洋大学, 辽宁 大连 116023; 2. 沈阳药科大学中药学院, 辽宁 沈阳 110016
  • 收稿日期:2011-07-30 修回日期:2011-08-28 出版日期:2011-09-28 发布日期:2011-10-28
  • 通讯作者: 谭成玉,博士,副教授. Tel: (0411)84763520
  • 基金资助:

    国家自然科学基金项目(21075012, 31071612)和辽宁省高等学校优秀人才支持计划项目(2009R13)

Isolation and analysis of a new phytoecdysteroid from Cyanotis arachnoidea C. B. Clarke

TAN Chengyu1*, KONG Liang1, LI Xi2, LI Wei1, LI Ning2   

  1. 1. Dalian Ocean University, Dalian 116023, China; 2. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2011-07-30 Revised:2011-08-28 Online:2011-09-28 Published:2011-10-28

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摘要： 云南植物露水草富含植物甾酮类成分。为了研究其中的生物活性成分,采用多种色谱分离方法(氧化铝柱色谱、硅胶柱色谱、常压反相C18柱色谱、制备薄层色谱及反相高效液相色谱等)相结合,从露水草中分离得到一种新植物甾酮。综合一维和二维核磁共振光谱以及电喷雾质谱数据分析,确定该化合物的结构为3β,4α,14α,20R,22R,25-hexahydroxy-5α-cholest-7-en-6-one,为一种较为少见的具有5α-H的植物甾酮。

关键词: 核磁共振光谱, 露水草, 色谱分离, 植物甾酮

Abstract: Cyanotis arachnoidea is a plant with plenty of phytoecdysteroid. To study the active compound in it, a new phytoecdysteroid with 5α-cholesta skeleton, was isolated from the whole plant of Cyanotis arachnoidea C. B. Clarke by using various chromatographic methods (alumina column chromatography, silica gel column chromatography, octadecyl silane (ODS) column chromatography, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)). Its structure was analyzed on the basis of 1D and 2D nuclear magnetic resonances (NMR), electrospray ionization mass spectrometry (ESI-MS) methods. It is a compound with structure of 3β,4α,14α,20R,22R,25-hexahydroxy-5α-cholest-7-en-6-one, which is a rare phytoecdysteroid with 5α-H.

Key words: Cyanotis arachnoidea C. B. Clarke, nuclear magnetic resonance (NMR), chromatographic isolation, phytoecdysteroid