印迹分子聚合物结合低共熔溶剂用于山楂中咖啡酸的固相萃取

doi:10.3724/SP.J.1123.2015.03025

色谱 ›› 2015, Vol. 33 ›› Issue (8): 792-798.DOI: 10.3724/SP.J.1123.2015.03025

• 特别策划:第20届全国色谱学术报告会专栏 • 上一篇下一篇

印迹分子聚合物结合低共熔溶剂用于山楂中咖啡酸的固相萃取

李桂珍¹, 唐为扬¹, 曹伟敏², 王倩¹, 朱涛¹

1. 天津市有机太阳能电池和光化学转换重点实验室, 天津理工大学化学化工学院, 天津 300384;
2. 石家庄以岭药业股份有限公司, 河北石家庄 050035

收稿日期:2015-03-19 出版日期:2015-08-08 发布日期:2015-07-27
通讯作者: 朱涛
基金资助:
This research was supported by Tianjin Municipal Natural Science Foundation of China (13JCYBJC42200) and Tianjin Municipal "131" Talents Program.

Molecularly imprinted polymers combination with deep eutectic solvents for solid-phase extraction of caffeic acid from hawthorn

LI Guizhen¹, TANG Weiyang¹, CAO Weimin², WANG Qian¹, ZHU Tao¹

1. Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China;
2. Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China

Received:2015-03-19 Online:2015-08-08 Published:2015-07-27
Supported by:
This research was supported by Tianjin Municipal Natural Science Foundation of China (13JCYBJC42200) and Tianjin Municipal "131" Talents Program.

摘要/Abstract

摘要：

在相同的实验条件下,分别合成了以咖啡酸为模板的印迹分子聚合物和无模板分子聚合物。使用场发射扫描电镜法和吸附实验表征这两种聚合物材料的孔状结构和选择性吸附性能。然后利用印迹分子聚合物、无模板分子聚合物、C₁₈萃取小柱这3种材料结合固相萃取法纯化山楂提取物中的咖啡酸,提取率分别为3.46、1.01、1.17 μg/g。为了优化固相萃取过程,实验研究了不同洗脱剂的影响。分别利用用氯化胆碱和甘油、氯化胆碱和尿素(摩尔比均为1:2)合成出两种低共熔溶剂。甲醇与这两种低共熔溶剂分别以不同的体积比混合作为洗脱剂,用于优化咖啡酸的固相萃取过程。实验结果表明,印迹分子聚合物是一种良好的固相萃取材料;当甲醇和甘油基低共熔溶剂在体积比为3:1混合时,表现出最好的洗脱能力,得到咖啡酸的回收率为82.32%。

关键词: 低共熔溶剂, 固相萃取法, 咖啡酸, 山楂, 印迹分子聚合物

Abstract:

Molecularly imprinted polymers (MIPs) with caffeic acid as template and non-imprinted polymers (NIPs) materials were prepared in the same procedure. Field emission scanning electron microscopy (FE-SEM) and adsorption capacity test were used to evaluate characteristic of the new materials. MIPs, NIPs and C₁₈ were used for rapid purification of caffeic acid from hawthorn with solid-phase extraction (SPE), and extract yields of caffeic acid with the proposed materials were 3.46 μg/g, 1.01 μg/g and 1.17 μg/g, respectively. To optimize the MIPs-SPE procedures, different kinds of elution solutions were studied. Deep eutectic solvents (DESs) were prepared by choline chloride (ChCl)-glycerol (1/2, n/n) and choline chloride-urea (1/2, n/n). Methanol was mixed with the two kinds of DESs (glycerol-based DESs, urea-based DESs) in different ratios (0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1, v/v), and they were used to investigated as elution solutions in the above MIPs-SPE procedures. The results showed that MIPs were potential SPE materials, and methanol/glycerol-based DESs (3:1, v/v) had the best elution capability with the recovery of 82.32%.

Key words: caffeic acid, deep eutectic solvents, hawthorn, molecularly imprinted polymers (MIPs), solid-phase extraction (SPE)

中图分类号:

O658

李桂珍, 唐为扬, 曹伟敏, 王倩, 朱涛. 印迹分子聚合物结合低共熔溶剂用于山楂中咖啡酸的固相萃取[J]. 色谱, 2015, 33(8): 792-798.

LI Guizhen, TANG Weiyang, CAO Weimin, WANG Qian, ZHU Tao. Molecularly imprinted polymers combination with deep eutectic solvents for solid-phase extraction of caffeic acid from hawthorn[J]. Chinese Journal of Chromatography, 2015, 33(8): 792-798.

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印迹分子聚合物结合低共熔溶剂用于山楂中咖啡酸的固相萃取

Molecularly imprinted polymers combination with deep eutectic solvents for solid-phase extraction of caffeic acid from hawthorn

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