色谱 ›› 2011, Vol. 29 ›› Issue (03): 244-248.DOI: 10.3724/SP.J.1123.2011.00244

• 研究论文 • 上一篇    下一篇

流动相离子色谱法同时测定植物中残留的矮壮素和缩节胺

周旭1,2, 许锦钢2, 陈智栋1, 朱岩2*   

  1. 1. 常州大学石油化工学院, 江苏 常州 213164; 2. 浙江大学化学系, 浙江 杭州 310028
  • 收稿日期:2010-12-06 修回日期:2011-01-09 出版日期:2011-03-28 发布日期:2011-03-28
  • 通讯作者: 朱岩,教授,主要研究方向为离子色谱.
  • 基金资助:

    国家自然科学基金(Nos. 20775070, J0830413)和浙江省自然科学基金(Nos. R4080124, Y4100190)项目.

Simultaneous determination of chlormequat chloride and mepiquat chloride in plants by mobile phase ion chromatography

ZHOU Xu1,2, XU Jingang2, CHEN Zhidong1, ZHU Yan2*   

  1. 1. School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China; 2. Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2010-12-06 Revised:2011-01-09 Online:2011-03-28 Published:2011-03-28
  • Contact:

摘要: 以离子对试剂作流动相,采用离子对抑制电导检测法同时测定植物中残留的矮壮素和缩节胺。简单处理后的样品经过Dionex IonPac NG1保护柱和NS1分离柱,在流速为1.00 mL/min、淋洗液为1.00 mmol/L九氟戊酸(作为离子对试剂)-7%(体积分数,下同)乙腈时等度洗脱分离,能够快速稳定出峰,且被测物与其他干扰离子充分分离。矮壮素和缩节胺的检出限分别为0.1546和0.1714 mg/L,在1~100 mg/L范围内具有良好的线性关系和重现性。对实际样品进行测定,矮壮素和缩节胺的回收率范围分别为96.06%~104.6%和98.53%~103.7%,相对标准偏差小于3%。该方法分析结果令人满意,可以满足矮壮素和缩节胺常规的定性、定量分析需求。

关键词: 矮壮素, 流动相离子色谱, 缩节胺, 抑制电导检测, 植物

Abstract: Ion-pair reagent was used as mobile phase for the simultaneous separation of chlormequat chloride and mepiquat chloride in plants. The rapid separation was performed on a Dionex IonPac NG1 guard column and a Dionex IonPac NS1 analytical column using 1.0 mL/min of the eluent mixture of 1.00 mmol/L nonafluoropentanoic acid (as ion-pair reagent) and 7% acetonitrile, and the detection was achieved by a suppressed conductivity detector. The method provided good resolution of the analyte peaks without any interference. The detection limits of chlormequat chloride and mepiquat chloride were 0.1546 mg/L and 0.1714 mg/L, respectively. The linear calibration curves were obtained in the range of 1~100 mg/L. The mean recoveries in the ranges of 96.06%~104.6% for chlormequat chloride and 98.53%~103.7% for mepiquat chloride were obtained in real samples. The method requires only simple sample preparation and the technique is suitable for routine quality control analysis.

Key words: chlormequat chloride, mepiquat chloride, plants, suppressed conductivity detection, mobile phase ion chromatography (MPIC)