色谱

色谱 ›› 2012, Vol. 30 ›› Issue (01): 103-106.DOI: 10.3724/SP.J.1123.2011.08034

• 技术与应用 • 上一篇    下一篇

毛细管电泳法测定注射用头孢哌酮钠他唑巴坦钠中目标成分

姜睿远, 孙国祥*   

  1. 沈阳药科大学药学院, 辽宁 沈阳 110016
  • 收稿日期:2011-08-22 修回日期:2011-09-27 出版日期:2012-01-28 发布日期:2012-03-01
  • 通讯作者: 孙国祥,博士,教授,博士生导师,研究方向为中药指纹学体系和其信息学及药物有关物质谱学. Tel: (024)23986286,

Determination of target compounds in cefoperazone sodium and tazobactam sodium for injection by capillary electrophoresis

JIANG Ruiyuan, SUN Guoxiang*   

  1. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2011-08-22 Revised:2011-09-27 Online:2012-01-28 Published:2012-03-01

PDF

331

被引次数

17

摘要： 建立了毛细管区带电泳法同时测定注射用头孢哌酮钠他唑巴坦钠中目标成分含量的方法。采用石英毛细管(75 cm×75 μm i.d.,有效长度63 cm),以40 mmol/L硼砂溶液为背景电解质(BGE),以氢氯噻嗪为内标,分离电压12.0 kV,检测波长220 nm,重力进样10 s(高度10 cm)。实验结果表明,头孢哌酮钠与他唑巴坦钠分别在0.25～3.96 g/L和0.062～0.99 g/L范围内呈良好的线性关系,相关系数(r)分别为0.9995和0.9996。精密度和重复性均良好,相对峰面积的相对标准偏差(RSD)均小于3%,制剂在208 min内稳定,回收试验结果符合方法学要求。该方法简单、准确、耗费少、污染小,可用于注射用粉针剂头孢哌酮钠他唑巴坦钠中头孢哌酮钠和他唑巴坦钠的定量分析。

关键词: 复方药, 毛细管电泳, 他唑巴坦钠, 头孢哌酮钠, 注射用头孢哌酮钠他唑巴坦钠

Abstract: A capillary zone electrophoresis (CZE) method was developed for the simultaneous determination of cefoperazone sodium and tazobactam sodium in the injectable powder of cefoperazone sodium and tazobactam sodium with hydrochlorothiazide as the internal standard. The operation was carried out on a quartz capillary (75 cm×75 μm i.d., 63 cm effective length). The electrophoretic conditions were as follows: 40 mmol/L borax solution as the back ground electrolyte (BGE), 12.0 kV applied voltage, 220 nm as the detection wavelength; the sample solution was injected by hydraulic pressure for 10 s at the height of 10 cm. The cefoperazone and tazobactam showed good linear relationship in the ranges of 0.25~3.96 g/L and 0.062~0.99 g/L with the correlation coefficients of 0.9995 and 0.9996, respectively. The relative standard deviations of relative peak areas were less than 3%. The preparation was stable in 208 min. The recovery results met the methodology requirements. The method is simple, rapid, reproducible, and suitable to control the quality of cefoperazone sodium and tazobactam sodium injectable powder.

Key words: cefoperazone sodium and tazobactam sodium for injection, compound drugs, tazobactam sodium, capillary electrophoresis （CE）, cefoperazone sodium