糖类化合物在两性离子亲水作用色谱柱上的保留行为评价

doi:10.3724/SP.J.1123.2015.05011

色谱 ›› 2015, Vol. 33 ›› Issue (9): 929-933.DOI: 10.3724/SP.J.1123.2015.05011

糖类化合物在两性离子亲水作用色谱柱上的保留行为评价

姜舸, 沈爱金, 郭志谋, 李秀玲, 梁鑫淼

中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁大连 116023

收稿日期:2015-05-11 出版日期:2015-09-08 发布日期:2015-09-09
通讯作者: 李秀玲
基金资助:
国家自然科学基金项目(21475129).

Evaluation of retention behavior of carbohydrate compounds on the zwitterionic hydrophilic interaction liquid chromatographic column

JIANG Ge, SHEN Aijin, GUO Zhimou, LI Xiuling, LIANG Xinmiao

Dalian Institute of Chemistry and Physics, Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China

Received:2015-05-11 Online:2015-09-08 Published:2015-09-09

摘要/Abstract

摘要：

糖类化合物因其极性强,在反相色谱模式下保留较弱,因此常用亲水作用色谱(HILIC)对其进行分离分析。本文以9种糖类化合物的混合物为研究对象,系统评价了其在Click TE-Cys亲水色谱柱上的保留行为,分别考察了流动相中有机相比例和盐浓度对其保留行为的影响。实验证明:9种糖类化合物按极性由小到大的顺序依次从Click TE-Cys色谱柱上被洗脱下来。随着有机相比例的增加,糖类化合物的保留增强;随着盐浓度的增加,除唾液酸外的糖类化合物的保留增强。用顶替-吸附液相相互作用模型模拟了糖类化合物在HILIC上的保留行为,采用保留方程ln k=a+blnC_B+cC_B描述HILIC的保留规律,对HILIC的保留值进行多元线性回归。结果表明糖类化合物在Click TE-Cys色谱柱上的保留行为符合HILIC的保留规律。

关键词: Click TE-Cys色谱柱, 保留行为, 两性离子固定相, 亲水作用色谱, 糖类化合物

Abstract:

Because of the strong polarity of carbohydrate compounds which have weak retention in reverse-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC) is suitable for the separation and analysis of the carbohydrate compounds. In this study, nine carbohydrate compounds were selected as the test probes to systematically evaluate the retention behavior of the carbohydrate compounds on a Click TE-Cys column with zwitterionic stationary phase. And the effects of the ratio of organic phase and salt concentration in mobile phase on the retention behavior were investigated as well. The experiments proved that the nine carbohydrate compounds could be eluted in the order of their polarities from the Click TE-Cys column. With the increase of organic phase ratio, the retention of carbohydrate compounds was enhanced. As the increase of salt concentration, the retention of the carbohydrate compounds increased except sialic acid. Using displacement-adsorption liquid phase interaction model to simulate the retention behavior of the carbohydrate compounds under HILIC, the retention behavior of HILIC was described by the retention equation of ln k=a+bln C_B+cC_B, and the retention values of HILIC were analyzed by multiple linear regression. The results demonstrated that the retention behavior of carbohydrate compounds on the Click TE-Cys was in accordance with the retention regularity of HILIC.

Key words: Click TE-Cys column, hydrophilic interaction liquid chromatography (HILIC), oligosaccharide compounds, retention behavior, zwitterionic stationary phase

中图分类号:

O658

姜舸, 沈爱金, 郭志谋, 李秀玲, 梁鑫淼. 糖类化合物在两性离子亲水作用色谱柱上的保留行为评价[J]. 色谱, 2015, 33(9): 929-933.

JIANG Ge, SHEN Aijin, GUO Zhimou, LI Xiuling, LIANG Xinmiao. Evaluation of retention behavior of carbohydrate compounds on the zwitterionic hydrophilic interaction liquid chromatographic column[J]. Chinese Journal of Chromatography, 2015, 33(9): 929-933.

参考文献

[1] Alpert A J. J Chromatogr A, 1990, 499: 177

[2] Fu Q, Wang J, Liang T, et al. Chinese Journal of Chromatography (傅青, 王军, 梁图, 等. 色谱), 2013, 31(11): 1051

[3] Jin X Q, Yao L X, Xue K P. Chemical Enterprise Management (金小青, 姚立新, 薛昆鹏. 化工管理), 2014, 27(9): 70

[4] Lin H, Feng Y, Yan C R, et al. Food and Drug (林慧, 冯云, 颜春荣, 等. 食品与药物), 2014, 16(6): 448

[5] Xue M, Lü Z, Wu Y, et al. Natural Product Research and Development (薛敏, 吕雉, 伍英, 等. 天然产物研究与开发), 2012, 24: 1715

[6] Leijdekkers A G M, Sanders M G, Schols H A, et al. J Chromatogr A, 2011, 1218(51): 9227

[7] Hernández-Hernández O, Calvillo I, Lebrón-Aguilar R, et al. J Chromatogr A, 2012, 1220: 57

[8] Liu T T, Zhao J. Chemical Research and Application (刘甜甜, 赵珺. 化学研究与应用), 2014, 26(5): 615

[9] Zhang L Y, Tian Y, Shen X H, et al. Journal of China Pharmaceutical University (张禄阳, 田媛, 沈晓航, 等. 中国药科大学学报), 2012, 43(4): 379

[10] Shen A J, Guo Z M, Cai X M, et al. J Chromatogr A, 2012, 1228: 175

[11] Shen A J, Guo Z M, Yu L, et al. Chem Commun, 2011, 47: 4550

[12] Jandera P. Anal Chim Acta, 2011, 692: 1

[13] Hemstrom P, Irgum K. J Sep Sci, 2006, 29: 1784

[14] Kumar A, Heaton J C, McCalley D V. J Chromatogr A, 2013, 1276: 33

[15] Han C, Dai X J, Lei G H, et al. Chinese Journal of Analytical Chemistry (韩超, 戴小军, 雷根虎, 等. 分析化学), 2010, 38(11): 1615

[16] McCalley D V. J Chromatogr A, 2007, 1171: 46

[17] Heaton J C, Russell J J, Underwood T, et al. J Chromatogr A, 2014, 1347: 39

[18] Li R P, Yuan Q, Zhang Y, et al. J Liq Chromatogr Rel Technol, 2011, 34: 511

[19] Dolan J W, Lommen D C, Snyder L R. J Chromatogr A, 1990, 535(1/2): 55

[20] Snyder L R, Poppe H. J Chromatogr A, 1980, 184(4): 363

[21] Nikitas P, Pappa-Louisi A, Agrafiotou P. J Chromatogr A, 2002, 946(1/2): 33

[22] Zou H F, Zhang Y K, Lu P Z. High Performance Liquid Chromatography. Beijing: Science Press (邹汉法, 张玉奎, 卢佩章. 高效液相色谱法. 北京: 科学出版社), 2001

糖类化合物在两性离子亲水作用色谱柱上的保留行为评价

Evaluation of retention behavior of carbohydrate compounds on the zwitterionic hydrophilic interaction liquid chromatographic column

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

扫码分享

参考文献

相关文章 15

编辑推荐

Metrics

[1]	刘小兰, 高薇, 梁超, 乔俊琴, 王康, 练鸿振. 缓冲盐类型和离子对试剂非对离子对强离解酸性化合物离子对反相液相色谱保留行为的影响[J]. 色谱, 2021, 39(9): 1021-1029.
[2]	刘小兰, 高薇, 梁超, 乔俊琴, 王康, 练鸿振. 硅胶基质固定相离子对反相液相色谱法测定强离解化合物的正辛醇/水分配系数[J]. 色谱, 2021, 39(11): 1230-1238.
[3]	宋春颖, 张华蓉, 郭志谋, 闫竞宇, 金高娃, 梁鑫淼. 固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量[J]. 色谱, 2020, 38(5): 547-553.
[4]	成晓东, 李云萍, 贺银菊. 一种可控离子比例的两性亲水色谱固定相的制备及性能研究[J]. 色谱, 2019, 37(7): 683-691.
[5]	魏莉莉, 薛霞, 刘艳明, 孙立臻, 程志, 宿书芳, 赵寅. 亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留[J]. 色谱, 2019, 37(7): 735-741.
[6]	李明昕, 王倩, 朱婧, 任琳, 雍莉, 邹晓莉. 亲水作用色谱-串联质谱测定尿液中尼古丁和可替宁[J]. 色谱, 2017, 35(8): 826-831.
[7]	李文婧, 赵丽娟, 魏缠玲, 戴小军, 龚波林. 磺酸甜菜碱型两性离子亲水作用色谱固定相的制备及色谱性能评价[J]. 色谱, 2017, 35(4): 382-387.
[8]	王惠, 辛华夏, 蔡剑锋, 李芳冰, 金郁, 傅青. 基于部分酸水解-亲水作用色谱的黄芪多糖指纹图谱分析及结合反相指纹图谱全面质量评价方法的建立(英文)[J]. 色谱, 2016, 34(7): 726-736.
[9]	用天, 吴凡, 肖红斌, 万伯顺. 木糖醇和麦芽糖醇型亲水作用色谱固定相的制备及其分离性能的评价[J]. 色谱, 2015, 33(9): 910-916.
[10]	成晓东, 冯钰锜. 三元醇丙烯酰胺键合色谱固定相的制备及评价[J]. 色谱, 2015, 33(9): 917-921.
[11]	谭婷, 乔鑫, 万益群, 邱洪灯. 低共熔溶剂——新型亲水作用色谱流动相改性剂[J]. 色谱, 2015, 33(9): 934-937.
[12]	龙珍, 金燕, 刘晓达, 郭志谋, 沈爱金, 胡兴娟, 吴宁鹏. 亲水作用色谱-电雾式检测用于5种抗病毒药物的定量分析[J]. 色谱, 2015, 33(9): 938-942.
[13]	刘艳明, 薛霞, 刘国强, 任雪梅, 胡梅, 祝建华. 亲水作用色谱-串联质谱法测定乳及乳制品中左旋肉碱[J]. 色谱, 2015, 33(9): 943-945.
[14]	朱银芳, 纪顺利, 李绍辉, 李成, 章飞芳, 梁鑫淼. 碳青霉烯类抗生素亲水作用色谱方法研究及其应用[J]. 色谱, 2015, 33(9): 946-950.
[15]	闫竞宇, 郭志谋, 丁俊杰, 沈爱金, 王纪霞, 金高娃, 梁鑫淼. 万古霉素及其杂质的亲水作用色谱分析[J]. 色谱, 2015, 33(9): 951-956.