超重现毛细管电泳的理论与例证

doi:10.3724/SP.J.1123.2020.07038

色谱 ›› 2020, Vol. 38 ›› Issue (10): 1217-1223.DOI: 10.3724/SP.J.1123.2020.07038

超重现毛细管电泳的理论与例证

陈义()

淮阴工学院, 江苏淮阴 223001; 中国科学院化学研究所, 中国科学院活体分析化学重点实验室, 北京 100190; 中国科学院大学, 北京 100049; 北京分子科学国家实验室, 北京 100190

收稿日期:2020-07-31 出版日期:2020-10-08 发布日期:2020-12-11
通讯作者: 陈义
作者简介:陈义.Tel:(010)62618240, E-mail:chenyi@iccas.ac.cn
基金资助:
国家自然科学基金项目(21727809);中国科学院项目(QYZDJ-SSW-SLH034)

Super stable capillary electrophoresis: theory and examples

CHEN Yi()

Huaiyin Institute of Technology, Huaiyin 223001, China; CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China; Beijing National Center for Molecular Sciences, Beijing 100190, China

Received:2020-07-31 Online:2020-10-08 Published:2020-12-11
Contact: CHEN Yi
Supported by:
National Natural Science Foundation of China(21727809);Project of Chinese Academy of Sciences(QYZDJ-SSW-SLH034)

摘要/Abstract

摘要：

毛细管电泳经常遭受结果不稳定、不重现的困扰。该文从理论上推导了一些新模式并以例证说明，这些模式能在一定条件下抵抗条件变化，获得超重现电泳谱图。它们是加权淌度谱、迁移电量谱、电荷密度谱、摩尔电荷密度谱、扩散系数谱、液相质量谱，以及它们各自的比例谱等，其中前4种为实时测量模式，其余的为实验后模式。这些模式需要发展新的仪器或算法，但都有发展潜力，值得深入研究。

关键词: 毛细管电泳, 非时间测量, 超重现模式, 理论推导, 例证

Abstract:

Capillary electrophoresis is often plagued by unstable and unrepeatable results. In this paper, some novel modes are derived theoretically and illustrated by separation examples, as expected these modes can resist the change of some conditions under certain prerequisites and obtain super stable electropherograms. They are weighted mobility spectrometry, migrated charge spectrometry, charge density spectrometry, molar charge density spectrometry, diffusion coefficient spectrometry, liquid-phase mass spectrometry, and their ratio spectrometry. The first four are real-time measurement modes while the others are post-experimental modes. These modes need to develop new devices and algorithm, but they are all highly promising and worth of further exploitation.

Key words: capillary electrophoresis (CE), non-time measurement, super stable model, theoretical deduction, examples

陈义. 超重现毛细管电泳的理论与例证[J]. 色谱, 2020, 38(10): 1217-1223.

CHEN Yi. Super stable capillary electrophoresis: theory and examples[J]. Chinese Journal of Chromatography, 2020, 38(10): 1217-1223.

图/表 6

图1 电场强度对CE-LIF之(a)时间、(b)加权淌度、(c)迁移电量和(d)电荷密度谱图的影响

Fig. 1 Impact of electric field strength on (a) time, (b) weighted mobility, (c) migrated charge and (d) charge density spectra measured by CE-LIF CE-LIF: capillary electrophoresis-laser-induced fluorescence detection. Capillary: 7 cm/13 cm (LR /total length)×50 μm ID; buffer: 5 mmol/L borax at pH 9.20; sample: fluorescein (FITC)-derived amino acids. The time spectra were measured in my lab by Dr. GUO Chao while the others were recalculated and replotted according to equation (12).

图2 (a) 时间、(b)加权淌度、(c)迁移电量和(d)电荷密度谱随毛细管和电场的变化

Fig. 2 Changes of (a) time, (b) weighted mobility, (c) migrated charge and (d) charge density spectra with capillary size and electric field The conditions are the same as in Fig. 1 .

图3 电泳缓冲液浓度对(a)加权淌度谱、(b)迁移电量谱、(c)电密度谱和(d)摩尔电密度谱的影响

Fig. 3 Impact of buffer concentration on (a) weighted mobility, (b) migrated charge, (c) charge density and (d) molar charge density spectra The other conditions are the same as in Fig. 1 .

图4 温度对迁移时间和迁移电荷密度的影响

Fig. 4 Changes of migration time and migrated charge density with temperature Capillary: 50 cm/56.8 cm (LR /total length)×50 μm ID; buffer: 3 mol/L acetic acid and 0.1% (w/v) hydroxyethyl cellulose; sample: 3.0 mg/mL human hemoglobin. The other conditions are the same as in Fig. 1 .

图5 混合样品测得的毛细管电泳扩散谱[22]

Fig. 5 Diffusion coefficient spectrum measured from a mixed sample by capillary electrophoresis[22] Capillary: 37 cm/55 cm (LR /total length)×75 μm ID; buffer: 10 mmol/L 3-(cyclohexylamino)-1-propanesulfonic acid at pH 9.00; voltage: 10 kV.

图6 聚乙二醇(5 kD)的(a)LMS和(b)MS谱图

Fig. 6 (a) LMS and (b) MS spectra of polyethylene glycol (5 kD) a. recalculated from the data in ref. [25]; b. replotted from ref. [25].

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超重现毛细管电泳的理论与例证

Super stable capillary electrophoresis: theory and examples

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