关键词:
stereoselective antibodies , chromatography, immunosensor, chiral separation

Abstract:

It has long been known that the configurational isomers of
biologically active compounds, e.g., nutrients, pesticides, and drugs,
may exhibit different activities in a chiral environment such as the
human body. Although the majority of drugs presently in development are
chiral, analytical and preparative methods for the quantitative
determination and purification of stereoisomers still lag behind. One
reason is that commonly used chiral selectors for the direct resolution
of enantiomers are not tailor-made for a specific analyte. The
identification of suitable selectors for a particular pair of
enantiomers still requires considerable experimentation and is generally
demanding with regard to material, time and labor. The rational design
of chiral host molecules, therefore, represents a challenge in
facilitating enantiomer analysis. In this article, we describe how a
combination of techniques ranging from organic synthesis to molecular
biology yields antibodies of predetermined specificity and
stereoselectivity that can be used as tailor-made chiral selectors for
the chromatographic separation of enantiomers and their sensitive
detection in immunosensors.

Key words:
stereoselective antibodies , chromatography, immunosensor, chiral separation