Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Isolation and Identification of the Mixture of L-Hydroxyproline
Oligo-Peptides by Reversed-Phase High Performance
Liquid Chromatography and Electrospray
Ionization Tandem Mass Spectrometry

SUN Yanting1, LU Kui1, MA Li1, CAO Shuxia2, ZHAO Yufen2,3   

  1. 1.School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450052, China;
    2.Key Laboratory for Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou
    450052, China; 3.Department of Chemistry, Xiamen University, Xiamen 361005, China
  • Received:2007-01-05 Revised:2007-02-12 Online:2007-07-30 Published:1985-06-25

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Abstract:

A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method （RP-HPLC/MS2） was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides. The mixture was separated on a YWG C8 column (10 μm, 250 mm×10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid (2∶98, v/v) as the mobile phase. The separation conditions were optimized. The mixture was analysed with electrospray ionization mass spectrometry （ESI-MS） and ESI-MS/MS in positive mode. MS and MS2 spectra of individual chromatographic peak were obtained. It indicated that there were hydroxyproline dipeptide, hydroxyproline tripeptide and hydroxyproline cyclo-dipeptide in the mixtures, besides hydroxyproline. The described method can be effectively applied to perform identification of hydroxyproline oligo-peptide mixtures.

Key words: electrospray ionization tandem mass spectrometry （ESI-MS/MS）, L-hydroxyproline oligo-peptide mixture , reversed-phase high performance liquid chromatography （RP-HPLC）