Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2008, Vol. 26 ›› Issue (3): 314-317.

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Rapid determination of alkaloids in tobacco using gas chromatography-mass spectrometry with full scan-selected ion monitoring mode

WANG Baoxing; YANG Shihua;HOU Ying;ZENG Xiaoying;WU Yi;XU Guowang   

  1. 1.Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming 650106, China;
    2.Hongyun Tobacco (Group) Co., Ltd, Kunming 650202, China;
    3.National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • Received:2007-08-15 Revised:2007-11-01 Online:2008-05-30 Published:1984-03-25

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Abstract: Simultaneous Scan-Selected ion monitoring mode (Scan-SIM) is a new improvement method of data acquisition of gas chromatography mass spectrometry (GC/MS). The determination method of alkaloids in tobacco was established by Scan-SIM of GC/MS. Nicotine, nornicotine, anabasine and anatabine were determined with Scan mode, and myosmine, nicotyrine, 2,3'-Bipyridyl and cotinine were determined with SIM mode. The average recoveries of alkaloids ranged from 94.8% to 98.8％ and relative standard deviations (RSDs) were less than 6.0% (n=5). Therefore, it is a simple, rapid, accurate method. Tobacco samples in different years were determined with this method, and the results were satisfactory.

Key words: alkaloids, full scan-selected ion monitoring mode （Scan-SIM）, gas chromatography-mass spectrometry （GC-MS）, tobacco