Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Establishment and Application of Two-dimensional Liquid Chromatography System(NPLC／RPLC)

LAN Tao1,2, JIAO Fenglong3, TANG Tao1,2, WANG Fengyun1, LI Tong2, ZHANG Weibing4   

  1. 1.Chemical Engineering Institute, Nanjing University of Science and Technology, Nanjing 210094, China;
    2.Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China; 3.Animal Science and
    Veterinary Institute, Jilin University, Changchun 130012, China; 4.Dalian Institute of
    Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • Received:2008-01-17 Revised:1900-01-01 Online:2008-05-30 Published:1984-03-25
  • Contact: ZHANG Weibing

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Abstract: A two-dimensional liquid chromatography (NPLC×RPLC) was developed. Normal phase liquid chromatography (NPLC) with a Hypersil SiO2 column (4.6mm×50 mm i. d.) was used as the first dimension, and reversed phase liquid chromatography (RPLC) with a Kromasil C18 column (4.6mm×250 mm i. d.) was used as the second dimension. The interface was based on a ten-port, two-position valve with a storage loop. As to improve the incompatibility of the two dimensional mobile phase and be good for adjusting the selectivity of separation, 1,4-Dioxane was used in the mobile phase of the first dimension, and isopropyl alcohol was used in the mobile phase of the second dimension based on the character of organic solvent. By rising the temperature of the second dimension, the system avoid the incompatibility of the two dimensional mobile phase. Zhengtian Wan was analyzed by this system, the total peak capacity reached 1120.

Key words: Sample loops-valve interface, Two-dimensional LC, Zhengtian Wan