Abstract: Preparative high performance liquid chromatography (HPLC) are widely used recently. The optimization of its operating conditions is important for improving efficiency and saving operating cost. Now, most works such as linear scale up technology were focused on the optimization of mobile phase. The sample collection region is another important operating parameter in preparative HPLC. In this study, a software was written according to the exponentially modified Gaussian （EMG） model. With this software, the operators of preparative HPLC can easily choose a suitable sample collection region.

Key words: collection region, optimization , preparative high performance liquid chromatography (HPLC), exponentially modified Gaussian （EMG） model