Abstract: A method was developed for the determination of benomyl, carbendazim and thiabendazole in apple juice concentrate by solid-phase extraction coupled with ion exchange chromatography (IEC). The sample was diluted with water, and then benomyl was degradated completely to carbendazim at 80 ℃, and purified by an SCX solid-phase extraction column. Liquid chromatographic analysis was performed on the instrument of Agilent 1200 series equipped with a diode-array detector and autosampler. The column was LC-SCX (25 cm×4.6 mm, 5 μm). The mobile phase was 0.1 mol/L KH2PO4 (pH 2.5)-acetonitrile (70∶30, v/v) with a flow rate of 1.0 mL/min. The presented method showed good linear relationship with good precision and accuracy at the range of 0.02-2.0 mg/L for carbendazim and hiabendazole. The detection limits were 0.004 mg/kg for carbendazim and thiabendazole. The method was characterized with cceptable sensitivity to meet the requirements for monitoring these pesticides in apple juice concentrate.

Key words: apple juice concentrate , benomyl, carbendazim, ion exchange chromatography （IEC）, thiabendazole, solid-phase extraction （SPE）