Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Isolation and analysis of a new phytoecdysteroid from Cyanotis arachnoidea C. B. Clarke

TAN Chengyu1*, KONG Liang1, LI Xi2, LI Wei1, LI Ning2   

  1. 1. Dalian Ocean University, Dalian 116023, China; 2. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2011-07-30 Revised:2011-08-28 Online:2011-09-28 Published:2011-10-28

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Abstract: Cyanotis arachnoidea is a plant with plenty of phytoecdysteroid. To study the active compound in it, a new phytoecdysteroid with 5α-cholesta skeleton, was isolated from the whole plant of Cyanotis arachnoidea C. B. Clarke by using various chromatographic methods (alumina column chromatography, silica gel column chromatography, octadecyl silane (ODS) column chromatography, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)). Its structure was analyzed on the basis of 1D and 2D nuclear magnetic resonances (NMR), electrospray ionization mass spectrometry (ESI-MS) methods. It is a compound with structure of 3β,4α,14α,20R,22R,25-hexahydroxy-5α-cholest-7-en-6-one, which is a rare phytoecdysteroid with 5α-H.

Key words: Cyanotis arachnoidea C. B. Clarke, nuclear magnetic resonance (NMR), chromatographic isolation, phytoecdysteroid