Abstract: A method of gas chromatography-ion trap mass spectrometry (GC-IT-MS) was developed for the determination of 21 fragrance allergens in sticker toys, plush toys and plastic toys. The experimental conditions, such as sample pretreatment conditions, and the analytical conditions of GC-IT-MS, were optimized. The sticker toy samples and plush toy samples were extracted with acetone by ultrasonic wave, and the extracts were separated on an Agilent HP-1MS column (50 m×0.2 mm×0.5 μm), then determined by IT-MS and quantified by external standard method. The plastic toy samples were extracted by the dissolution-precipitation approach, cleaned up with an Envi-carb solid phase extraction column and concentrated by rotary evaporation and nitrogen blowing, then determined by GC-IT-MS and quantified by external standard method. The calibration curves showed good linearity in the range of 0.002~50 mg/L with the correlation coefficients greater than 0.9968. The limits of quantification (LOQ, S/N＞10) were 0.02~40 mg/kg. The average recoveries of the target compounds spiked in the sample at three concentration levels were in the range of 82.2%~110.8% with the relative standard deviations (RSDs) of 0.6%~10.5%. These results show that this method is accurate and sensitive for the qualitative and quantitative determination of the 21 fragrance allergens in the 3 types of toys.

Key words: fragrance allergens, toys, gas chromatography-ion trap mass spectrometry (GC-IT-MS)